BARD Glossary

 

ID URI Term Description Units External Ontology References
3 http://www.bard.nih.gov/ontology/bard#BARD_0000001 assay protocol BARD> assay protocol
Experimental protocol designed to test the effect of a perturbagen on a biological entity, measuring one or more assay readouts, facilitated by an assay design and assay type, and recording the results as one or more endpoints that quantify or qualify the extent of perturbation.
Open Biomedical Ontologies : http://purl.obolibrary.org/obo/ID_0000004
4 http://www.bard.nih.gov/ontology/bard#BARD_0000002 assay component BARD> assay protocol> assay component
Physical entity participating in the assay reaction, common across all reactions in the assay (with the exception of a control that may be in specified reactions), but not including the perturbagen; the purpose of each component is defined by the assay component role.
Open Biomedical Ontologies : http://purl.obolibrary.org/obo/ID_0000008
9 http://www.bard.nih.gov/ontology/bard#BARD_0000007 assay component concentration BARD> assay protocol> assay component> assay component concentration
The final concentration of the specified assay component following addition of all assay components, at which the relevant reaction or measurement occurs.
Open Biomedical Ontologies : http://purl.obolibrary.org/obo/ID_0000373
1945 http://www.bard.nih.gov/ontology/bard#BARD_0001827 assay component concentration (% (mass)) BARD> assay protocol> assay component> assay component concentration> assay component concentration (% (mass))
% (mass)
1946 http://www.bard.nih.gov/ontology/bard#BARD_0001828 assay component concentration (% (vol)) BARD> assay protocol> assay component> assay component concentration> assay component concentration (% (vol))
% (vol)
2368 http://bard.broadinstitute.org/bard#2368 assay component concentration (OD600) BARD> assay protocol> assay component> assay component concentration> assay component concentration (OD600)
Optical density of a component (e.g. bacterial culture) suspension at 600 nm
1947 http://www.bard.nih.gov/ontology/bard#BARD_0001829 assay component concentration (W/V) BARD> assay protocol> assay component> assay component concentration> assay component concentration (W/V)
ug/mL
2302 http://bard.broadinstitute.org/bard#2302 assay component concentration (cells/container) BARD> assay protocol> assay component> assay component concentration> assay component concentration (cells/container)
#/well
2304 http://bard.broadinstitute.org/bard#2304 assay component concentration (cells/volume) BARD> assay protocol> assay component> assay component concentration> assay component concentration (cells/volume)
cells/mL
1944 http://www.bard.nih.gov/ontology/bard#BARD_0001826 assay component concentration (molar) BARD> assay protocol> assay component> assay component concentration> assay component concentration (molar)
uM
10 http://www.bard.nih.gov/ontology/bard#BARD_0000008 assay component name BARD> assay protocol> assay component> assay component name
11 http://www.bard.nih.gov/ontology/bard#BARD_0000009 assay component role BARD> assay protocol> assay component> assay component role
Defines the purpose of an assay component in a specific assay definition, i.e. target, substrate, measured component, etc. For example, a protein might have an assay compontent role of target in one assay definition, and in another it would have the role of coupled enzyme.
59 http://www.bard.nih.gov/ontology/bard#BARD_0000057 application role BARD> assay protocol> assay component> assay component role> application role
Open Biomedical Ontologies - CHEBI : http://purl.obolibrary.org/obo/CHEBI_33232
150 http://www.bard.nih.gov/ontology/bard#BARD_0000148 assay control role BARD> assay protocol> assay component> assay component role> assay control role
A compound that is routinely run in the same manner as the test compounds in every run of the assay. This term does not refer to the plate controls used to define the maximum and minimum responses, and it may or may not be a literature standard or reference compound.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000072
229 http://www.bard.nih.gov/ontology/bard#BARD_0000227 background control BARD> assay protocol> assay component> assay component role> assay control role> background control
The readings obtained from wells to which the sample or one of the reagents was not added. This is subtracted from all the experimental readings prior to further analysis. Depending on the composition of the inhibitor stocks, DMSO might be needed in the control wells to assure consistency across all the experiments.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000334
230 http://www.bard.nih.gov/ontology/bard#BARD_0000228 high-signal control BARD> assay protocol> assay component> assay component role> assay control role> high-signal control
Contains the substrate titration without inhibitor to reflect the maximum enzyme activity at each substrate concentration. Depending on the composition of the inhibitor stocks, DMSO might be needed in the control wells to assure consistency across all the experiments.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000156
231 http://www.bard.nih.gov/ontology/bard#BARD_0000229 low-signal control BARD> assay protocol> assay component> assay component role> assay control role> low-signal control
Contains the substrate titration without enzyme or substrate and without inhibitor. The low controls should reflect the signal expected for no enzyme activity at each substrate concentration. Depending on the composition of the inhibitor stocks, DMSO might be needed in the control wells to assure consistency across all the experiments.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000168
232 http://www.bard.nih.gov/ontology/bard#BARD_0000230 negative control BARD> assay protocol> assay component> assay component role> assay control role> negative control
Used to determine the baseline against which the effect of the test perturbagen is compared. Often the negative control is the solvent (e.g., DMSO) in which the perturbagen was dissolved.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000079
233 http://www.bard.nih.gov/ontology/bard#BARD_0000231 positive control BARD> assay protocol> assay component> assay component role> assay control role> positive control
A chemical compound or reagent used in each plate of an assay to normalize the response of the test perturbagens (by plate). The positive control is known from previous experiments or is a previously established standard. It is usually highly active, resulting in a strong response of the intended effect. In an inhibition assay, the positive control would usually result in the complete inhibition, which measurement is then used for normalization. In an activation assay it would result in high activation, which measurement is then used for normalization. Using controls provides an external reference and reduces the number of false negatives and false positives.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000080
61 http://www.bard.nih.gov/ontology/bard#BARD_0000059 biological role BARD> assay protocol> assay component> assay component role> biological role
An assay component role that plays the role of the primary biological focal point of the assay.
Open Biomedical Ontologies - CHEBI : http://purl.obolibrary.org/obo/CHEBI_24432
151 http://www.bard.nih.gov/ontology/bard#BARD_0000149 cell-culture role BARD> assay protocol> assay component> assay component role> cell-culture role
249 http://www.bard.nih.gov/ontology/bard#BARD_0000247 antibiotic BARD> assay protocol> assay component> assay component role> cell-culture role> antibiotic
An assay component role that is a substance used to kill or inhibit the growth of a microorganism, esp. bacteria. May also be used as a detection method in recombinant plasmid DNA production
Open Biomedical Ontologies - CHEBI : http://purl.obolibrary.org/obo/CHEBI_22582
250 http://www.bard.nih.gov/ontology/bard#BARD_0000248 differentiation agent BARD> assay protocol> assay component> assay component role> cell-culture role> differentiation agent
An assay component role which is used in cell-culture protocols in order to cease cell proliferation and induce physiological changes in the cell to produce the final cell type for a tissue or organ.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002087
251 http://www.bard.nih.gov/ontology/bard#BARD_0000249 fixative BARD> assay protocol> assay component> assay component role> cell-culture role> fixative
An assay component role used in cell-culture protocols as a means to preserve the cell to resemble a living state as closely as possible.
Open Biomedical Ontologies - CHEBI : http://purl.obolibrary.org/obo/CHEBI_50913
252 http://www.bard.nih.gov/ontology/bard#BARD_0000250 growth factor BARD> assay protocol> assay component> assay component role> cell-culture role> growth factor
A cell-culture role that is a substance used in the culture medium to stimulate cellular growth, usually a protein or growth hormone.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002024
253 http://www.bard.nih.gov/ontology/bard#BARD_0000251 media component BARD> assay protocol> assay component> assay component role> cell-culture role> media component
A cell-culture role that is a substance or reagent used as part of the culture medium.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002102
254 http://www.bard.nih.gov/ontology/bard#BARD_0000252 transfection agent BARD> assay protocol> assay component> assay component role> cell-culture role> transfection agent
A cell-culture reagent which is used to introduce nucleic acids of interest into cells, esp. animal cells.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002105
1488 http://www.bard.nih.gov/ontology/bard#BARD_0001613 untreated BARD> assay protocol> assay component> assay component role> cell-culture role> untreated
1681 http://www.bard.nih.gov/ontology/bard#BARD_0001647 chaperone BARD> assay protocol> assay component> assay component role> chaperone
62 http://www.bard.nih.gov/ontology/bard#BARD_0000060 chemical role BARD> assay protocol> assay component> assay component role> chemical role
Open Biomedical Ontologies - CHEBI : http://purl.obolibrary.org/obo/CHEBI_51086
152 http://www.bard.nih.gov/ontology/bard#BARD_0000150 detection role BARD> assay protocol> assay component> assay component role> detection role
A type of assay component thats purpose is to allow for detection of a measurable effect in the substance or tissue of study.
256 http://www.bard.nih.gov/ontology/bard#BARD_0000254 analyte BARD> assay protocol> assay component> assay component role> detection role> analyte
A chemical substance which is analyzed through measurement of its chemical properties in order to examine the effects of a perturbagen.
1420 http://www.bard.nih.gov/ontology/bard#BARD_0001595 cofactor BARD> assay protocol> assay component> assay component role> detection role> cofactor
257 http://www.bard.nih.gov/ontology/bard#BARD_0000255 dye BARD> assay protocol> assay component> assay component role> detection role> dye
An assay component role which serves a detection role due to its affinity to the substance or tissue to which is binds and the resulting color change of the substance or tissue.
Open Biomedical Ontologies - CHEBI : http://purl.obolibrary.org/obo/CHEBI_37958
258 http://www.bard.nih.gov/ontology/bard#BARD_0000256 measured component BARD> assay protocol> assay component> assay component role> detection role> measured component
A detection role that defines a non-specific, measurable substance used in the experimental protocol to detect a particular endpoint.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002001
259 http://www.bard.nih.gov/ontology/bard#BARD_0000257 radioactive label BARD> assay protocol> assay component> assay component role> detection role> radioactive label
Open Biomedical Ontologies - CHEBI : http://purl.obolibrary.org/obo/CHEBI_35211
260 http://www.bard.nih.gov/ontology/bard#BARD_0000258 reference BARD> assay protocol> assay component> assay component role> detection role> reference
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002092
261 http://www.bard.nih.gov/ontology/bard#BARD_0000259 reporter BARD> assay protocol> assay component> assay component role> detection role> reporter
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0003061
1457 http://www.bard.nih.gov/ontology/bard#BARD_0001606 single-feature extraction BARD> assay protocol> assay component> assay component role> detection role> single-feature extraction
used in Broad, AID 2632
262 http://www.bard.nih.gov/ontology/bard#BARD_0000260 stain BARD> assay protocol> assay component> assay component role> detection role> stain
263 http://www.bard.nih.gov/ontology/bard#BARD_0000261 substrate BARD> assay protocol> assay component> assay component role> detection role> substrate
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0003063
1458 http://www.bard.nih.gov/ontology/bard#BARD_0001437 tracer BARD> assay protocol> assay component> assay component role> detection role> tracer
used in Broad, AID 2632
153 http://www.bard.nih.gov/ontology/bard#BARD_0000151 modulator role BARD> assay protocol> assay component> assay component role> modulator role
321 http://www.bard.nih.gov/ontology/bard#BARD_0000319 activator BARD> assay protocol> assay component> assay component role> modulator role> activator
Any compound which functions to initiate or increase the effects of a molecule or biological action such as increasing the rate of an enzymatic reaction, positively regulating gene expression through increased rate of transcription, or a similar mechanism.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002088
322 http://www.bard.nih.gov/ontology/bard#BARD_0000320 agonist BARD> assay protocol> assay component> assay component role> modulator role> agonist
An assay component role describing a compound that binds to a cell's receptor and triggers a response.
Open Biomedical Ontologies - CHEBI : http://purl.obolibrary.org/obo/CHEBI_48705
323 http://www.bard.nih.gov/ontology/bard#BARD_0000321 antagonist BARD> assay protocol> assay component> assay component role> modulator role> antagonist
An assay component role describing a compound that binds to a receptor and blocks its function or blocks the function of an agonist.
Open Biomedical Ontologies - CHEBI : http://purl.obolibrary.org/obo/CHEBI_48706
324 http://www.bard.nih.gov/ontology/bard#BARD_0000322 blocker BARD> assay protocol> assay component> assay component role> modulator role> blocker
a substance or drug which inhibits a certain response from occurring by binding to an active site or receptor and preventing other biological molecules from interacting with that site
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002085
325 http://www.bard.nih.gov/ontology/bard#BARD_0000323 competitor BARD> assay protocol> assay component> assay component role> modulator role> competitor
Cold ligand is an unlabeled chemical entity (such as a hormone or neurotransmitter) that is used to compete with an identical labeled chemical (radioligand) in binding to a specific receptor in a homologous competitive binding assay.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0001056
326 http://www.bard.nih.gov/ontology/bard#BARD_0000324 depolarizer BARD> assay protocol> assay component> assay component role> modulator role> depolarizer
An assay component role which is a substance used to depolarize an electrochemical cell by changing the polarity across the cell membrane, making the cell more positive.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002098
1313 http://www.bard.nih.gov/ontology/bard#BARD_0001580 fixed cell BARD> assay protocol> assay component> assay component role> modulator role> fixed cell
327 http://www.bard.nih.gov/ontology/bard#BARD_0000325 inducer BARD> assay protocol> assay component> assay component role> modulator role> inducer
A modulator that functions to activate gene expression by either binding to and disabling a repressor or binding to an activator.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0003058
328 http://www.bard.nih.gov/ontology/bard#BARD_0000326 inhibitor BARD> assay protocol> assay component> assay component role> modulator role> inhibitor
A modulator that functions to suppress or reduce the activity of a biomolecule or biological process
Open Biomedical Ontologies - CHEBI : http://purl.obolibrary.org/obo/CHEBI_35222
329 http://www.bard.nih.gov/ontology/bard#BARD_0000327 inverse agonist BARD> assay protocol> assay component> assay component role> modulator role> inverse agonist
A modulator that binds to the same receptor as the agonist but induces a response opposite to that of the agonist.
330 http://www.bard.nih.gov/ontology/bard#BARD_0000328 modulator BARD> assay protocol> assay component> assay component role> modulator role> modulator
331 http://www.bard.nih.gov/ontology/bard#BARD_0000329 partial agonist BARD> assay protocol> assay component> assay component role> modulator role> partial agonist
Chemical molecule that binds to a receptor and induce a response that has decreased efficacy relative to the full agonist.
332 http://www.bard.nih.gov/ontology/bard#BARD_0000330 perturbagen BARD> assay protocol> assay component> assay component role> modulator role> perturbagen
A reagent introduced to cause a disruption in the normal activity of the biological target.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0003059
333 http://www.bard.nih.gov/ontology/bard#BARD_0000331 potentiator BARD> assay protocol> assay component> assay component role> modulator role> potentiator
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0003060
334 http://www.bard.nih.gov/ontology/bard#BARD_0000332 sensitizer BARD> assay protocol> assay component> assay component role> modulator role> sensitizer
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0003062
1315 http://www.bard.nih.gov/ontology/bard#BARD_0001582 stable BARD> assay protocol> assay component> assay component role> modulator role> stable
1715 http://www.bard.nih.gov/ontology/bard#BARD_0001654 probe BARD> assay protocol> assay component> assay component role> probe
154 http://www.bard.nih.gov/ontology/bard#BARD_0000152 reagent role BARD> assay protocol> assay component> assay component role> reagent role
340 http://www.bard.nih.gov/ontology/bard#BARD_0000338 assay medium BARD> assay protocol> assay component> assay component role> reagent role> assay medium
The cell culture broth used while performing an assay on cells, which is optimized for each assay type. Some interfering additives such as serum, growth factors, buffers, amino acids, antibiotics, etc., might be eliminated in this medium.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000596
341 http://www.bard.nih.gov/ontology/bard#BARD_0000339 buffer BARD> assay protocol> assay component> assay component role> reagent role> buffer
An assay component that is an aqueous solution of either weak acid and its conjugate base or weak base and its conjugate acid; it is used to maintain pH stability in a solution by preventing protonation of basic compounds or preventng deprotonation of acidic compounds. Examples include Tris and HEPES
Open Biomedical Ontologies - CHEBI : http://purl.obolibrary.org/obo/CHEBI_35225
342 http://www.bard.nih.gov/ontology/bard#BARD_0000340 carrier BARD> assay protocol> assay component> assay component role> reagent role> carrier
An assay component role that is a molecule that transports or facilitates transport of another molecule
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002093
343 http://www.bard.nih.gov/ontology/bard#BARD_0000341 coupled enzyme BARD> assay protocol> assay component> assay component role> reagent role> coupled enzyme
A type of assay component role which is an enzyme that is paired to the reaction of another enzyme that can be detected more easily; used to study the function of an enzyme whose activity is not as easily detected alone.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002096
344 http://www.bard.nih.gov/ontology/bard#BARD_0000342 coupled substrate BARD> assay protocol> assay component> assay component role> reagent role> coupled substrate
A type of assay component role which is a substrate that is paired to the reaction of another substrate that can be detected more easily; used to study the function of an enzyme or substrate whose activity is not as easily detected alone.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002030
345 http://www.bard.nih.gov/ontology/bard#BARD_0000343 cross-linking reagent BARD> assay protocol> assay component> assay component role> reagent role> cross-linking reagent
A type of assay component role which describes a substance used to form artificial bridges between polymer chains either to induce changes in a molecules physical properties or to link proteins together.
Open Biomedical Ontologies - CHEBI : http://purl.obolibrary.org/obo/CHEBI_50684
346 http://www.bard.nih.gov/ontology/bard#BARD_0000344 detergent BARD> assay protocol> assay component> assay component role> reagent role> detergent
A type of assay component role which is a reagent that acts as a surfactant, generally serving as a cleaning agent.
Open Biomedical Ontologies - CHEBI : http://purl.obolibrary.org/obo/CHEBI_27780
347 http://www.bard.nih.gov/ontology/bard#BARD_0000345 ligand BARD> assay protocol> assay component> assay component role> reagent role> ligand
An assay reagent that binds to and forms a complex with another molecule for a given purpose.
Open Biomedical Ontologies - CHEBI : http://purl.obolibrary.org/obo/CHEBI_52214
348 http://www.bard.nih.gov/ontology/bard#BARD_0000346 reagent BARD> assay protocol> assay component> assay component role> reagent role> reagent
Open Biomedical Ontologies - CHEBI : http://purl.obolibrary.org/obo/CHEBI_33893
349 http://www.bard.nih.gov/ontology/bard#BARD_0000347 reducing agent BARD> assay protocol> assay component> assay component role> reagent role> reducing agent
Open Biomedical Ontologies - CHEBI : http://purl.obolibrary.org/obo/CHEBI_63247
350 http://www.bard.nih.gov/ontology/bard#BARD_0000348 solute BARD> assay protocol> assay component> assay component role> reagent role> solute
A reagent that serves as the substance that is dissolved with a solvent to form a solution, a homogenous mixture of a single phase.
BioAssay Ontology v2 : http://www.bioassayontology.org/bao2.0_development#BAO_0002103
351 http://www.bard.nih.gov/ontology/bard#BARD_0000349 solvent BARD> assay protocol> assay component> assay component role> reagent role> solvent
A reagent that serves to dissolve a solute and results in a solution, a homogenous mixture of a single phase.
Open Biomedical Ontologies - CHEBI : http://purl.obolibrary.org/obo/CHEBI_46787
352 http://www.bard.nih.gov/ontology/bard#BARD_0000350 vehicle BARD> assay protocol> assay component> assay component role> reagent role> vehicle
An assay component that serves as a carrier of a desired substance, typically a drug, to the target subject.
BioAssay Ontology v2 : http://www.bioassayontology.org/bao2.0_development#BAO_0002106
155 http://www.bard.nih.gov/ontology/bard#BARD_0000153 subject role BARD> assay protocol> assay component> assay component role> subject role
364 http://www.bard.nih.gov/ontology/bard#BARD_0000362 animal subject BARD> assay protocol> assay component> assay component role> subject role> animal subject
A whole, living organism that is used to assess the effects of a drug (efficacy, toxicity, etc), disease, or some other measured parameter on a living organism
1792 http://www.bard.nih.gov/ontology/bard#BARD_0001688 antitarget BARD> assay protocol> assay component> assay component role> subject role> antitarget
365 http://www.bard.nih.gov/ontology/bard#BARD_0000363 host cell BARD> assay protocol> assay component> assay component role> subject role> host cell
A subject role that is the biological structure which houses the infectious agent or perturbagen in the experimental protocol.
366 http://www.bard.nih.gov/ontology/bard#BARD_0000364 infectious agent BARD> assay protocol> assay component> assay component role> subject role> infectious agent
A subject that functions to carry and induce an infection into the cell or organism into which it is introduced.
367 http://www.bard.nih.gov/ontology/bard#BARD_0000365 receptor BARD> assay protocol> assay component> assay component role> subject role> receptor
368 http://www.bard.nih.gov/ontology/bard#BARD_0000366 target BARD> assay protocol> assay component> assay component role> subject role> target
An assay component role that is the particular macromolecule or gene which is the main focus of the experimental protocols of the assay definition, as in what is being perturbed in order to measure an effect on that particular assay component
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0003064
369 http://www.bard.nih.gov/ontology/bard#BARD_0000367 target cell BARD> assay protocol> assay component> assay component role> subject role> target cell
An assay component role that is the particular cell or cell-line which is the main focus of the experimental protocols of the assay definition, as in what is being perturbed in order to measure an effect on that particular assay component
1574 http://www.bard.nih.gov/ontology/bard#BARD_0001631 transcription factor BARD> assay protocol> assay component> assay component role> transcription factor
12 http://www.bard.nih.gov/ontology/bard#BARD_0000010 assay component type BARD> assay protocol> assay component> assay component type
A term which provides a general description of the assay component, for example whether it is a biological component or small-molecule component.
Open Biomedical Ontologies : http://purl.obolibrary.org/obo/ID_0000372
63 http://www.bard.nih.gov/ontology/bard#BARD_0000061 assay kit name BARD> assay protocol> assay component> assay component type> assay kit name
Defined set of commercially available or well known materials, reagents and proven protocols needed for an assay to deliver concise results in the least amount of time. However, the specific molecular components may or may not be known (as in the case of proprietary kit components).
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000248
2334 http://bard.broadinstitute.org/bard#2334 AB Power SYBR Green BARD> assay protocol> assay component> assay component type> assay kit name> AB Power SYBR Green
664 http://www.bard.nih.gov/ontology/bard#BARD_0000669 ACTOne Membrane Potential Dye Kit BARD> assay protocol> assay component> assay component type> assay kit name> ACTOne Membrane Potential Dye Kit
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000172
666 http://www.bard.nih.gov/ontology/bard#BARD_0000671 ADP Hunter Plus Kit BARD> assay protocol> assay component> assay component type> assay kit name> ADP Hunter Plus Kit
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000815
667 http://www.bard.nih.gov/ontology/bard#BARD_0000672 ADP-Glo Kinase Assay BARD> assay protocol> assay component> assay component type> assay kit name> ADP-Glo Kinase Assay
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000690
669 http://www.bard.nih.gov/ontology/bard#BARD_0000674 ATPlite Luminescence Assay System BARD> assay protocol> assay component> assay component type> assay kit name> ATPlite Luminescence Assay System
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000689
2356 http://bard.broadinstitute.org/bard#2356 spectrophotometer BARD> assay protocol> assay component> assay component type> assay kit name> ATPlite Luminescence Assay System> spectrophotometer
665 http://www.bard.nih.gov/ontology/bard#BARD_0000670 Adapta Universal Kinase Assay Kit BARD> assay protocol> assay component> assay component type> assay kit name> Adapta Universal Kinase Assay Kit
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000814
1773 http://www.bard.nih.gov/ontology/bard#BARD_0001674 Alpco insulin ELISA assay kit BARD> assay protocol> assay component> assay component type> assay kit name> Alpco insulin ELISA assay kit
1632 http://www.bard.nih.gov/ontology/bard#BARD_0001109 AlphaScreen Beads BARD> assay protocol> assay component> assay component type> assay kit name> AlphaScreen Beads
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002725
1966 http://bard.broadinstitute.org/bard#1966 Alphalisa Bead kit BARD> assay protocol> assay component> assay component type> assay kit name> Alphalisa Bead kit
PerkinElmer Alphalisa fluoescent bead kit
668 http://www.bard.nih.gov/ontology/bard#BARD_0000673 Amplex Red Glucos/Glucose Assay Kit BARD> assay protocol> assay component> assay component type> assay kit name> Amplex Red Glucos/Glucose Assay Kit
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000816
2335 http://bard.broadinstitute.org/bard#2335 AnaSpec Enzolyte 520 Protease Assay Kit BARD> assay protocol> assay component> assay component type> assay kit name> AnaSpec Enzolyte 520 Protease Assay Kit
2319 http://bard.broadinstitute.org/bard#2319 Apo-ONE(R) Homogeneous Caspase-3-7 BARD> assay protocol> assay component> assay component type> assay kit name> Apo-ONE(R) Homogeneous Caspase-3-7
Caspase assay detection kit
1633 http://www.bard.nih.gov/ontology/bard#BARD_0001110 BODIPY Nucleotide Analogue BARD> assay protocol> assay component> assay component type> assay kit name> BODIPY Nucleotide Analogue
BioAssay Ontology v2 : http://www.bioassayontology.org/bao2.0_development#BAO_0000817
1634 http://www.bard.nih.gov/ontology/bard#BARD_0001111 BTC AM Fluorescent Dye Indicator BARD> assay protocol> assay component> assay component type> assay kit name> BTC AM Fluorescent Dye Indicator
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000870
670 http://www.bard.nih.gov/ontology/bard#BARD_0000675 BacTiter-Glo Microbial Cell Viability Assay BARD> assay protocol> assay component> assay component type> assay kit name> BacTiter-Glo Microbial Cell Viability Assay
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000688
671 http://www.bard.nih.gov/ontology/bard#BARD_0000676 Beta-Glo Assay System BARD> assay protocol> assay component> assay component type> assay kit name> Beta-Glo Assay System
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000687
672 http://www.bard.nih.gov/ontology/bard#BARD_0000677 Bright-Glo Luciferase Assay System BARD> assay protocol> assay component> assay component type> assay kit name> Bright-Glo Luciferase Assay System
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000685
2339 http://bard.broadinstitute.org/bard#2339 BugLite BARD> assay protocol> assay component> assay component type> assay kit name> BugLite
2001 http://bard.broadinstitute.org/bard#2001 CDP-star BARD> assay protocol> assay component> assay component type> assay kit name> CDP-star
674 http://www.bard.nih.gov/ontology/bard#BARD_0000679 Calcium Assay Kit BARD> assay protocol> assay component> assay component type> assay kit name> Calcium Assay Kit
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000873
676 http://www.bard.nih.gov/ontology/bard#BARD_0000681 Caspase-Glo 3/7 Assay System BARD> assay protocol> assay component> assay component type> assay kit name> Caspase-Glo 3/7 Assay System
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000683
2383 http://bard.broadinstitute.org/bard#2383 Cell to CT RT kit BARD> assay protocol> assay component> assay component type> assay kit name> Cell to CT RT kit
Ambion
677 http://www.bard.nih.gov/ontology/bard#BARD_0000682 CellTiter-Glo Luminescent Cell Viability Assay BARD> assay protocol> assay component> assay component type> assay kit name> CellTiter-Glo Luminescent Cell Viability Assay
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000682
678 http://www.bard.nih.gov/ontology/bard#BARD_0000683 Chroma-Glo Luciferase Assay System BARD> assay protocol> assay component> assay component type> assay kit name> Chroma-Glo Luciferase Assay System
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000681
1860 http://www.bard.nih.gov/ontology/bard#BARD_0001745 CytoTox-ONE Homogenous Membrane Integrity Assay BARD> assay protocol> assay component> assay component type> assay kit name> CytoTox-ONE Homogenous Membrane Integrity Assay
2345 http://bard.broadinstitute.org/bard#2345 Cytotox-Glo Luciferase Assay System BARD> assay protocol> assay component> assay component type> assay kit name> Cytotox-Glo Luciferase Assay System
2347 http://bard.broadinstitute.org/bard#2347 DUB-Glo Protease Assay BARD> assay protocol> assay component> assay component type> assay kit name> DUB-Glo Protease Assay
a homogeneous, bioluminescent assay that measures the activity of numerous deconjugating enzymes including deubiquitinating (DUB), deSUMOylating (SENP) and deneddylating (NEDP) proteases. These proteases reverse the protein modification by ubiquitin and ubiquitin-like proteins (Ubl proteins) and thus are integral components in the complex mechanisms of posttranslational protein regulation in eukaryotes.
2346 http://bard.broadinstitute.org/bard#2346 DiscoverX b-Arrestin Detection Reagents BARD> assay protocol> assay component> assay component type> assay kit name> DiscoverX b-Arrestin Detection Reagents
679 http://www.bard.nih.gov/ontology/bard#BARD_0000684 Dual-Luciferase Reporter Assay System BARD> assay protocol> assay component> assay component type> assay kit name> Dual-Luciferase Reporter Assay System
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000680
1864 http://www.bard.nih.gov/ontology/bard#BARD_0001748 Duke Scientific 4 um bead BARD> assay protocol> assay component> assay component type> assay kit name> Duke Scientific 4 um bead
63
1635 http://www.bard.nih.gov/ontology/bard#BARD_0001112 ELISA cAMP Competition Assay using cAMP-Screen Direct System BARD> assay protocol> assay component> assay component type> assay kit name> ELISA cAMP Competition Assay using cAMP-Screen Direct System
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002727
2348 http://bard.broadinstitute.org/bard#2348 EnzChek Phosphate Assay Kit BARD> assay protocol> assay component> assay component type> assay kit name> EnzChek Phosphate Assay Kit
1636 http://www.bard.nih.gov/ontology/bard#BARD_0001516 Fluo-4 AM Calcium Indicator BARD> assay protocol> assay component> assay component type> assay kit name> Fluo-4 AM Calcium Indicator
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000907
2300 http://bard.broadinstitute.org/bard#2300 Fluo-4 NW Calcium Assay Kit BARD> assay protocol> assay component> assay component type> assay kit name> Fluo-4 NW Calcium Assay Kit
Assay Kit that uses Fluo-4 as a detector of Calcium Flux. A no wash modification of the original Fluo-4 AM Calcium Assay Kit
1637 http://www.bard.nih.gov/ontology/bard#BARD_0001517 Fluo-8 Calcium Assay Kit BARD> assay protocol> assay component> assay component type> assay kit name> Fluo-8 Calcium Assay Kit
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002672
680 http://www.bard.nih.gov/ontology/bard#BARD_0000685 Fluo-8 No Wash Calcium Assay Kit BARD> assay protocol> assay component> assay component type> assay kit name> Fluo-8 No Wash Calcium Assay Kit
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000908
1638 http://www.bard.nih.gov/ontology/bard#BARD_0001518 FluoZin BARD> assay protocol> assay component> assay component type> assay kit name> FluoZin
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000916
1639 http://www.bard.nih.gov/ontology/bard#BARD_0001519 FluoZin-2 BARD> assay protocol> assay component> assay component type> assay kit name> FluoZin-2
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000915
681 http://www.bard.nih.gov/ontology/bard#BARD_0000686 FluxOR Thallium Detection Kit BARD> assay protocol> assay component> assay component type> assay kit name> FluxOR Thallium Detection Kit
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000917
2003 http://bard.broadinstitute.org/bard#2003 Fura-2-AM BARD> assay protocol> assay component> assay component type> assay kit name> Fura-2-AM
fluorescent indicator
1869 http://www.bard.nih.gov/ontology/bard#BARD_0001753 GalScreen base kit BARD> assay protocol> assay component> assay component type> assay kit name> GalScreen base kit
682 http://www.bard.nih.gov/ontology/bard#BARD_0000687 GeneBLAzer BARD> assay protocol> assay component> assay component type> assay kit name> GeneBLAzer
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000923
2351 http://bard.broadinstitute.org/bard#2351 Glosensor BARD> assay protocol> assay component> assay component type> assay kit name> Glosensor
1456 http://www.bard.nih.gov/ontology/bard#BARD_0001108 HDAC fluorometric detection assay kit BARD> assay protocol> assay component> assay component type> assay kit name> HDAC fluorometric detection assay kit
686 http://www.bard.nih.gov/ontology/bard#BARD_0000691 HTRF KinEASE TK assay system BARD> assay protocol> assay component> assay component type> assay kit name> HTRF KinEASE TK assay system
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000939
684 http://www.bard.nih.gov/ontology/bard#BARD_0000689 HTRF cAMP HiRange Kit BARD> assay protocol> assay component> assay component type> assay kit name> HTRF cAMP HiRange Kit
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000938
685 http://www.bard.nih.gov/ontology/bard#BARD_0000690 HTRF cAMP Kit BARD> assay protocol> assay component> assay component type> assay kit name> HTRF cAMP Kit
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000937
687 http://www.bard.nih.gov/ontology/bard#BARD_0000692 HTScan IKK-beta Kinase Assay Kit BARD> assay protocol> assay component> assay component type> assay kit name> HTScan IKK-beta Kinase Assay Kit
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000940
688 http://www.bard.nih.gov/ontology/bard#BARD_0000693 HTScan PKC-beta Kinase Assay Kit BARD> assay protocol> assay component> assay component type> assay kit name> HTScan PKC-beta Kinase Assay Kit
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000941
689 http://www.bard.nih.gov/ontology/bard#BARD_0000694 HTScan PKC-theta Kinase Assay Kit BARD> assay protocol> assay component> assay component type> assay kit name> HTScan PKC-theta Kinase Assay Kit
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000942
683 http://www.bard.nih.gov/ontology/bard#BARD_0000688 High Performance Calcium Assay Kit BARD> assay protocol> assay component> assay component type> assay kit name> High Performance Calcium Assay Kit
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000936
1434 http://www.bard.nih.gov/ontology/bard#BARD_0001107 Human cytochrome c ELISA kit BARD> assay protocol> assay component> assay component type> assay kit name> Human cytochrome c ELISA kit
from the Broad - Patrick AID min Assay Sheet
1402 http://www.bard.nih.gov/ontology/bard#BARD_0001106 IMAP Kinase assay kit BARD> assay protocol> assay component> assay component type> assay kit name> IMAP Kinase assay kit
690 http://www.bard.nih.gov/ontology/bard#BARD_0000695 IP-One HTRF Assay BARD> assay protocol> assay component> assay component type> assay kit name> IP-One HTRF Assay
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000948
1640 http://www.bard.nih.gov/ontology/bard#BARD_0001520 Indo-1 Calcium Indicator BARD> assay protocol> assay component> assay component type> assay kit name> Indo-1 Calcium Indicator
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000945
691 http://www.bard.nih.gov/ontology/bard#BARD_0000696 Kinase-Glo Luminescent Kinase Assay BARD> assay protocol> assay component> assay component type> assay kit name> Kinase-Glo Luminescent Kinase Assay
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000679
692 http://www.bard.nih.gov/ontology/bard#BARD_0000697 Kinase-Glo Plus Luminescent Kinase Assay BARD> assay protocol> assay component> assay component type> assay kit name> Kinase-Glo Plus Luminescent Kinase Assay
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000678
693 http://www.bard.nih.gov/ontology/bard#BARD_0000698 LANCE cAMP Detection Kit BARD> assay protocol> assay component> assay component type> assay kit name> LANCE cAMP Detection Kit
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000953
2384 http://bard.broadinstitute.org/bard#2384 Light Cycler Probes Master BARD> assay protocol> assay component> assay component type> assay kit name> Light Cycler Probes Master
Roche
694 http://www.bard.nih.gov/ontology/bard#BARD_0000699 LiveBLAzer-FRET B/G Loading Kit BARD> assay protocol> assay component> assay component type> assay kit name> LiveBLAzer-FRET B/G Loading Kit
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000958
2330 http://bard.broadinstitute.org/bard#2330 Luciferin detection reagent (Promega) BARD> assay protocol> assay component> assay component type> assay kit name> Luciferin detection reagent (Promega)
2015 http://bard.broadinstitute.org/bard#2015 Membrane Potential Assay Kit BARD> assay protocol> assay component> assay component type> assay kit name> Membrane Potential Assay Kit
Molecular Devices Membrane Potential Assay Kit
1896 http://www.bard.nih.gov/ontology/bard#BARD_0001777 Membrane Potential Assay Kit Blue BARD> assay protocol> assay component> assay component type> assay kit name> Membrane Potential Assay Kit Blue
1872 http://www.bard.nih.gov/ontology/bard#BARD_0001755 Millipore HDAC Assay Kit BARD> assay protocol> assay component> assay component type> assay kit name> Millipore HDAC Assay Kit
Millipore/Upstate HDAC Assay Kit (fluormetric detection)
695 http://www.bard.nih.gov/ontology/bard#BARD_0000700 ONE-Glo Luciferase Assay System BARD> assay protocol> assay component> assay component type> assay kit name> ONE-Glo Luciferase Assay System
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000675
696 http://www.bard.nih.gov/ontology/bard#BARD_0000701 OptEIA Human IL-8 ELISA Set BARD> assay protocol> assay component> assay component type> assay kit name> OptEIA Human IL-8 ELISA Set
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000983
2333 http://bard.broadinstitute.org/bard#2333 Orange TranscreenerTM ADP2 BARD> assay protocol> assay component> assay component type> assay kit name> Orange TranscreenerTM ADP2
697 http://www.bard.nih.gov/ontology/bard#BARD_0000702 P450-Glo CYP1A2 Screening System BARD> assay protocol> assay component> assay component type> assay kit name> P450-Glo CYP1A2 Screening System
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000674
698 http://www.bard.nih.gov/ontology/bard#BARD_0000703 P450-Glo CYP2C19 Screening System BARD> assay protocol> assay component> assay component type> assay kit name> P450-Glo CYP2C19 Screening System
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000670
699 http://www.bard.nih.gov/ontology/bard#BARD_0000704 P450-Glo CYP2C9 Screening System BARD> assay protocol> assay component> assay component type> assay kit name> P450-Glo CYP2C9 Screening System
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000333
700 http://www.bard.nih.gov/ontology/bard#BARD_0000705 P450-Glo CYP2D6 Screening System BARD> assay protocol> assay component> assay component type> assay kit name> P450-Glo CYP2D6 Screening System
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000311
701 http://www.bard.nih.gov/ontology/bard#BARD_0000706 P450-Glo CYP3A4 Screening System BARD> assay protocol> assay component> assay component type> assay kit name> P450-Glo CYP3A4 Screening System
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000310
704 http://www.bard.nih.gov/ontology/bard#BARD_0000709 PKLight Assay Kit BARD> assay protocol> assay component> assay component type> assay kit name> PKLight Assay Kit
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000307
702 http://www.bard.nih.gov/ontology/bard#BARD_0000707 PathHunter beta-Arrestin Assay BARD> assay protocol> assay component> assay component type> assay kit name> PathHunter beta-Arrestin Assay
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000988
1957 http://bard.broadinstitute.org/bard#1957 PerkinElmer AlphaScreen Bead Kit BARD> assay protocol> assay component> assay component type> assay kit name> PerkinElmer AlphaScreen Bead Kit
AlphaScreen protein-protein interaction assay
1890 http://www.bard.nih.gov/ontology/bard#BARD_0001771 PerkinElmer Alphalisa Bead kit BARD> assay protocol> assay component> assay component type> assay kit name> PerkinElmer Alphalisa Bead kit
PerkinElmer Alphalisa fluoescent bead kit
703 http://www.bard.nih.gov/ontology/bard#BARD_0000708 Phospho(Thr180/Tyr182)/Total p38 Assay Whole Cell Lysate Kit BARD> assay protocol> assay component> assay component type> assay kit name> Phospho(Thr180/Tyr182)/Total p38 Assay Whole Cell Lysate Kit
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0001049
1997 http://bard.broadinstitute.org/bard#1997 Polar Screen PPARg Competitor Assay BARD> assay protocol> assay component> assay component type> assay kit name> Polar Screen PPARg Competitor Assay
63
705 http://www.bard.nih.gov/ontology/bard#BARD_0000710 Proteasome-Glo Chymotrypsine-Like Assay BARD> assay protocol> assay component> assay component type> assay kit name> Proteasome-Glo Chymotrypsine-Like Assay
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000299
2354 http://bard.broadinstitute.org/bard#2354 ProteoStat PDI Assay Kit BARD> assay protocol> assay component> assay component type> assay kit name> ProteoStat PDI Assay Kit
2355 http://bard.broadinstitute.org/bard#2355 Q5 Real Time PCR Detection system BARD> assay protocol> assay component> assay component type> assay kit name> Q5 Real Time PCR Detection system
2018 http://bard.broadinstitute.org/bard#2018 RNeasy Qiagen BARD> assay protocol> assay component> assay component type> assay kit name> RNeasy Qiagen
RNA isolation kit
1884 http://www.bard.nih.gov/ontology/bard#BARD_0001765 Renilla Glo luciferase assay BARD> assay protocol> assay component> assay component type> assay kit name> Renilla Glo luciferase assay
1998 http://bard.broadinstitute.org/bard#1998 Roche FuGene6 BARD> assay protocol> assay component> assay component type> assay kit name> Roche FuGene6
DNA Transfection Agent
2043 http://bard.broadinstitute.org/bard#2043 SYBR green fluorescent dye BARD> assay protocol> assay component> assay component type> assay kit name> SYBR green fluorescent dye
2325 http://bard.broadinstitute.org/bard#2325 Sensolyte 520 MMP-2 Assay Kit BARD> assay protocol> assay component> assay component type> assay kit name> Sensolyte 520 MMP-2 Assay Kit
706 http://www.bard.nih.gov/ontology/bard#BARD_0000711 Steady-Glo Luciferase Assay System BARD> assay protocol> assay component> assay component type> assay kit name> Steady-Glo Luciferase Assay System
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000298
1464 http://www.bard.nih.gov/ontology/bard#BARD_0001608 SuperSignal ELISA Femto Luminol/Enhancer Solution BARD> assay protocol> assay component> assay component type> assay kit name> SuperSignal ELISA Femto Luminol/Enhancer Solution
used in Broad AID # 485416
2366 http://bard.broadinstitute.org/bard#2366 SureLight Allophycocyanin BARD> assay protocol> assay component> assay component type> assay kit name> SureLight Allophycocyanin
2331 http://bard.broadinstitute.org/bard#2331 SureLight Allopohycocyanin BARD> assay protocol> assay component> assay component type> assay kit name> SureLight Allopohycocyanin
1842 http://www.bard.nih.gov/ontology/bard#BARD_0001729 Sybr Green PCR Reagent Kit BARD> assay protocol> assay component> assay component type> assay kit name> Sybr Green PCR Reagent Kit
708 http://www.bard.nih.gov/ontology/bard#BARD_0000713 Tango GPCR Assay System BARD> assay protocol> assay component> assay component type> assay kit name> Tango GPCR Assay System
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0001025
2538 http://bard.broadinstitute.org/bard#2538 The Renilla-Glo Luciferase Assay System BARD> assay protocol> assay component> assay component type> assay kit name> The Renilla-Glo Luciferase Assay System
The Renilla-Glo Luciferase Assay System is a single-addition reagent that generates a glow-type signal with Renilla luciferase. When reconstituted, it has the capacity to lyse cells, reduce the autoluminescence of the coelenterazine substrate, and produce a stable signal (i.e., half-life greater than 60 minutes at 22 degC).
2358 http://bard.broadinstitute.org/bard#2358 Trizol total RNA extraction kit BARD> assay protocol> assay component> assay component type> assay kit name> Trizol total RNA extraction kit
2359 http://bard.broadinstitute.org/bard#2359 Ub-CHOP2-Reporter-Kit BARD> assay protocol> assay component> assay component type> assay kit name> Ub-CHOP2-Reporter-Kit
1641 http://www.bard.nih.gov/ontology/bard#BARD_0001521 Vybrant MTT Cell Proliferation Assay Kit BARD> assay protocol> assay component> assay component type> assay kit name> Vybrant MTT Cell Proliferation Assay Kit
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002728
2038 http://bard.broadinstitute.org/bard#2038 WST-1 Cell Proliferation Assay Kit BARD> assay protocol> assay component> assay component type> assay kit name> WST-1 Cell Proliferation Assay Kit
2336 http://bard.broadinstitute.org/bard#2336 aScript One-Step Fast qRT-PCR lit BARD> assay protocol> assay component> assay component type> assay kit name> aScript One-Step Fast qRT-PCR lit
673 http://www.bard.nih.gov/ontology/bard#BARD_0000678 britelite plus Reporter Gene Assay System BARD> assay protocol> assay component> assay component type> assay kit name> britelite plus Reporter Gene Assay System
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000686
675 http://www.bard.nih.gov/ontology/bard#BARD_0000680 cAMP-Screen Direct System BARD> assay protocol> assay component> assay component type> assay kit name> cAMP-Screen Direct System
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000898
707 http://www.bard.nih.gov/ontology/bard#BARD_0000712 steadylite HTS Reagent BARD> assay protocol> assay component> assay component type> assay kit name> steadylite HTS Reagent
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000216
709 http://www.bard.nih.gov/ontology/bard#BARD_0000714 xMAP Kit BARD> assay protocol> assay component> assay component type> assay kit name> xMAP Kit
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002018
64 http://www.bard.nih.gov/ontology/bard#BARD_0000062 assay reagent name BARD> assay protocol> assay component> assay component type> assay reagent name
Reagent added to an assay that is not defined as a kit, biological entity, or small molecule (e.g., common buffer mixtures).
Open Biomedical Ontologies : http://purl.obolibrary.org/obo/ID_0000021
2332 http://bard.broadinstitute.org/bard#2332 Biomol Green reagent BARD> assay protocol> assay component> assay component type> assay reagent name> Biomol Green reagent
1939 http://www.bard.nih.gov/ontology/bard#BARD_0001820 CellTiter 96 Reagent BARD> assay protocol> assay component> assay component type> assay reagent name> CellTiter 96 Reagent
2344 http://bard.broadinstitute.org/bard#2344 CellTiter-Fluor BARD> assay protocol> assay component> assay component type> assay reagent name> CellTiter-Fluor
2341 http://bard.broadinstitute.org/bard#2341 ColorLock Gold BARD> assay protocol> assay component> assay component type> assay reagent name> ColorLock Gold
2306 http://bard.broadinstitute.org/bard#2306 FLIPR Membrane Potential Blue BARD> assay protocol> assay component> assay component type> assay reagent name> FLIPR Membrane Potential Blue
membrane potential sensing dye
2311 http://bard.broadinstitute.org/bard#2311 Fluo-4 BARD> assay protocol> assay component> assay component type> assay reagent name> Fluo-4
Fluo-4 Invitrogen
2353 http://bard.broadinstitute.org/bard#2353 Luciferase detection reagent BARD> assay protocol> assay component> assay component type> assay reagent name> Luciferase detection reagent
1881 http://www.bard.nih.gov/ontology/bard#BARD_0001763 SigmaFast protease inhibitor substrate BARD> assay protocol> assay component> assay component type> assay reagent name> SigmaFast protease inhibitor substrate
1879 http://www.bard.nih.gov/ontology/bard#BARD_0001761 ThioGlo-1 detection reagent BARD> assay protocol> assay component> assay component type> assay reagent name> ThioGlo-1 detection reagent
ThioGlo-1 thiol detection reagent
65 http://www.bard.nih.gov/ontology/bard#BARD_0000063 biological component BARD> assay protocol> assay component> assay component type> biological component
Assay component type that describes the material entity of biological origin (e.g., protein, cell culture, tissue).
Open Biomedical Ontologies : http://purl.obolibrary.org/obo/ID_0000022
162 http://www.bard.nih.gov/ontology/bard#BARD_0000160 biological component type BARD> assay protocol> assay component> assay component type> biological component> biological component type
243 http://www.bard.nih.gov/ontology/bard#BARD_0000241 biological fluid BARD> assay protocol> assay component> assay component type> biological component> biological component type> biological fluid
Open Biomedical Ontologies : http://purl.obolibrary.org/obo/ID_0000471
1508 http://www.bard.nih.gov/ontology/bard#BARD_0001448 brain BARD> assay protocol> assay component> assay component type> biological component> biological component type> biological fluid> brain
1424 http://www.bard.nih.gov/ontology/bard#BARD_0001597 human plasma BARD> assay protocol> assay component> assay component type> biological component> biological component type> biological fluid> human plasma
from UNM min Assay annnotaion xls
1432 http://www.bard.nih.gov/ontology/bard#BARD_0001602 mouse plasma BARD> assay protocol> assay component> assay component type> biological component> biological component type> biological fluid> mouse plasma
from UNM min Assay annotation xls
1465 http://www.bard.nih.gov/ontology/bard#BARD_0001609 platelet-rich plasma BARD> assay protocol> assay component> assay component type> biological component> biological component type> biological fluid> platelet-rich plasma
used as an "assay component type" to describe a CID # in AID# 488895, 504843, 504941. Think It is really a name not a component type.
244 http://www.bard.nih.gov/ontology/bard#BARD_0000242 cultured cell BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell
Open Biomedical Ontologies : http://purl.obolibrary.org/obo/ID_0000266
1542 http://www.bard.nih.gov/ontology/bard#BARD_0001627 cell modification temperature BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cell modification temperature
deg C
375 http://www.bard.nih.gov/ontology/bard#BARD_0000373 cell-culture condition BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cell-culture condition
Open Biomedical Ontologies : http://purl.obolibrary.org/obo/ID_0000128
430 http://www.bard.nih.gov/ontology/bard#BARD_0000429 cell-culture component BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cell-culture condition> cell-culture component
Components of a specific medium in which a cell culture is prepared, which is optimized for its growth; includes medium additives such as serum, growth factors, buffers, amino acids, and antibiotics (this information may be obtained from ATCC or found in relevant publications).
Open Biomedical Ontologies : http://purl.obolibrary.org/obo/ID_0000130
478 http://www.bard.nih.gov/ontology/bard#BARD_0000478 culture additive BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cell-culture condition> cell-culture component> culture additive
Open Biomedical Ontologies : http://purl.obolibrary.org/obo/ID_0000394
479 http://www.bard.nih.gov/ontology/bard#BARD_0000479 culture antibiotic BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cell-culture condition> cell-culture component> culture antibiotic
Open Biomedical Ontologies : http://purl.obolibrary.org/obo/ID_0000395
480 http://www.bard.nih.gov/ontology/bard#BARD_0000480 culture medium BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cell-culture condition> cell-culture component> culture medium
The liquid broth used to grow cells, which is optimized for each cell type and includes additives such as growth factors, buffers, amino acids, antibiotics, etc. This information can be obtained from ATCC or found in relevant publications.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000114
481 http://www.bard.nih.gov/ontology/bard#BARD_0000481 culture serum BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cell-culture condition> cell-culture component> culture serum
Serum containing growth factors required for cultured cells division, optimized for each cell culture; typically, fetal bovine serum is used, but other sera such horse serum are used (specialized assay sera, such as dextran charcoal-treated or dialyzed sera, used at the assay stage to avoid interference from normal serum components, should be specified as 'assay reagent').
Open Biomedical Ontologies : http://purl.obolibrary.org/obo/ID_0000327
431 http://www.bard.nih.gov/ontology/bard#BARD_0000430 cell-growth mode BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cell-culture condition> cell-growth mode
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002099
484 http://www.bard.nih.gov/ontology/bard#BARD_0000484 adherent BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cell-culture condition> cell-growth mode> adherent
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002101
485 http://www.bard.nih.gov/ontology/bard#BARD_0000485 mixed BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cell-culture condition> cell-growth mode> mixed
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002107
486 http://www.bard.nih.gov/ontology/bard#BARD_0000486 suspension BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cell-culture condition> cell-growth mode> suspension
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002104
432 http://www.bard.nih.gov/ontology/bard#BARD_0000431 passage number BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cell-culture condition> passage number
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002788
433 http://www.bard.nih.gov/ontology/bard#BARD_0000432 tissue-culture flask name BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cell-culture condition> tissue-culture flask name
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002192
862 http://www.bard.nih.gov/ontology/bard#BARD_0000819 HYPERflask BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cell-culture condition> tissue-culture flask name> HYPERflask
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002520
863 http://www.bard.nih.gov/ontology/bard#BARD_0000820 T175 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cell-culture condition> tissue-culture flask name> T175
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002517
864 http://www.bard.nih.gov/ontology/bard#BARD_0000821 T225 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cell-culture condition> tissue-culture flask name> T225
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002514
865 http://www.bard.nih.gov/ontology/bard#BARD_0000822 T25 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cell-culture condition> tissue-culture flask name> T25
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002199
866 http://www.bard.nih.gov/ontology/bard#BARD_0000823 T75 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cell-culture condition> tissue-culture flask name> T75
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002506
867 http://www.bard.nih.gov/ontology/bard#BARD_0000824 TripleFlask BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cell-culture condition> tissue-culture flask name> TripleFlask
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002521
376 http://www.bard.nih.gov/ontology/bard#BARD_0000374 cell-line modification method BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cell-line modification method
Describes the type of alterations performed on the cell line, which include plasmid transfection, viral transduction, cell fusion, etc.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000238
434 http://www.bard.nih.gov/ontology/bard#BARD_0000433 bacterial infection method BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cell-line modification method> bacterial infection method
Live cell assay in which eukaryotic cells are infected with bacteria.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000547
435 http://www.bard.nih.gov/ontology/bard#BARD_0000434 cell-fusion method BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cell-line modification method> cell-fusion method
Method by which a hybrid cell is formed from two different cells of same or different species. After such fusion, one of the parental cells has a dominant phenotype over the other, and the resulting hybrids will have the features of only one of the parental cells. Fusion is performed to understand the dominance of genetic material, DNA recombination events, etc.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000240
482 http://www.bard.nih.gov/ontology/bard#BARD_0000482 cybrid cell creation method BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cell-line modification method> cell-fusion method> cybrid cell creation method
Technique that creates a cybrid cell by fusing a nucleated and an enucleated (nucleus removed) cell. The resulting cybrids have one nucleus, but they contain the mitochondria from both the parental cells. This allows one to study the mitochondrial inheritance and to ascertain the role of mitochondrial DNA mutations in diseases.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000242
483 http://www.bard.nih.gov/ontology/bard#BARD_0000483 hybrid cell creation method BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cell-line modification method> cell-fusion method> hybrid cell creation method
Cell fusion is the formation of a hybrid cell from two different cells of same or different species. Fusion hybrids are created by fusing two nucleated cells obtained from same or different species.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000243
436 http://www.bard.nih.gov/ontology/bard#BARD_0000435 genetic modification method BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cell-line modification method> genetic modification method
Cloned genes are introduced into cells by various methods, including transfection, transduction, electroporation, nucleofection, etc. It is generally performed to express the exogenously introduced gene and study its effect on the cell. In addition, DNA encoding regulatory moieties, such as siRNA, is similarly introduced to inhibit the expression of a specific gene(s).
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000241
504 http://www.bard.nih.gov/ontology/bard#BARD_0000504 transfection method BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cell-line modification method> genetic modification method> transfection method
Technology that introduces nucleic acids into cells using a construct. Plasmids constructs are created by the insertion of a gene or siRNA-encoding DNA of interest into a plasmid. The engineered plasmids are introduced into cells by various means, including transfection, electroporation, and nucleofection, that regulate the efficiency and location of gene transfer.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000245
527 http://www.bard.nih.gov/ontology/bard#BARD_0000527 stable transfection method BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cell-line modification method> genetic modification method> transfection method> stable transfection method
Technology in which the introduced plasmid construct is stably integrated in the cellular genome, and is therefore a permanent modification of the cells. This is achieved by selecting for cells that express a plasmid-encoded selectable marker, which is commonly an antibiotic resistance protein.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000246
528 http://www.bard.nih.gov/ontology/bard#BARD_0000528 transient transfection method BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cell-line modification method> genetic modification method> transfection method> transient transfection method
Technology in which a gene or siRNA encoding DNA of interest is temporarily introduced into a cell using various plasmids. Usually, the transiently transfected DNA remains in the cell for 2-3 days and is functional, expressing the gene of interest. There are numerous transient transfection reagents and protocols available that have been optimized for various cell lines.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000247
505 http://www.bard.nih.gov/ontology/bard#BARD_0000505 viral transduction method BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cell-line modification method> genetic modification method> viral transduction method
Technology in which cells are infected by a pathogenic or a modified virus, which introduces viral genetic material into the cells. Pathogenic viruses used include the influenza virus, measles virus, and Japanese encephalitis virus. For recombinant expression of proteins in cells or tissues, several types of virus are utilized, including lentivirus, retrovirus, and adenovirus, with each having their own advantages and disadvantages.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000244
529 http://www.bard.nih.gov/ontology/bard#BARD_0000529 pathogenic virus transduction method BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cell-line modification method> genetic modification method> viral transduction method> pathogenic virus transduction method
Technology in which cells are infected with a disease-causing virus that could affect humans, animals, and plants. Examples of pathogenic viruses include the influenza virus, measles virus, and Japanese encephalitis virus.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000546
530 http://www.bard.nih.gov/ontology/bard#BARD_0000530 recombinant virus transduction method BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cell-line modification method> genetic modification method> viral transduction method> recombinant virus transduction method
Technology in which cells are infected with a modified virus that introduces a viral genome containing foreign genes. Several types of viruses are used for recombinant gene expression, including lentivirus, retrovirus, adenovirus, etc., and each has its own advantages and disadvantages.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000545
1953 http://www.bard.nih.gov/ontology/bard#BARD_0000375 cultured cell name BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name
An assay component type that describes the name of the cultured cell used in the experiment.
1952 http://www.bard.nih.gov/ontology/bard#BARD_0001834 ATCC cell name BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> ATCC cell name
Uses ATCC lookup
377 http://www.bard.nih.gov/ontology/bard#BARD_0001825 other cell name BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name
To be seeded with data from ATCC. Uses ATCC lookup (from UNM min Assay annnotaion xls)
Open Biomedical Ontologies : http://purl.obolibrary.org/obo/ID_0000025
711 http://www.bard.nih.gov/ontology/bard#BARD_0000716 293 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> 293
Open Biomedical Ontologies - CLO : http://purl.obolibrary.org/obo/CLO_0001230
712 http://www.bard.nih.gov/ontology/bard#BARD_0000717 293T/17 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> 293T/17
Open Biomedical Ontologies - CLO : http://purl.obolibrary.org/obo/CLO_0001235
2291 http://bard.broadinstitute.org/bard#2291 697 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> 697
Human
2297 http://bard.broadinstitute.org/bard#2297 70Z3 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> 70Z3
1930 http://www.bard.nih.gov/ontology/bard#BARD_0001811 786 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> 786
786 Renal Cancer cell line
2416 http://bard.broadinstitute.org/bard#2416 786-0 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> 786-0
2420 http://bard.broadinstitute.org/bard#2420 A-C/EBP 3 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> A-C/EBP 3
2421 http://bard.broadinstitute.org/bard#2421 A-CREB 1 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> A-CREB 1
2422 http://bard.broadinstitute.org/bard#2422 A-CREB 2 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> A-CREB 2
2423 http://bard.broadinstitute.org/bard#2423 A-FOS 2 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> A-FOS 2
2424 http://bard.broadinstitute.org/bard#2424 A-FOS 3 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> A-FOS 3
2425 http://bard.broadinstitute.org/bard#2425 A-JUN 1 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> A-JUN 1
2426 http://bard.broadinstitute.org/bard#2426 A-JUN 3 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> A-JUN 3
2417 http://bard.broadinstitute.org/bard#2417 A204/ATCC BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> A204/ATCC
1880 http://www.bard.nih.gov/ontology/bard#BARD_0001762 A375 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> A375
A375 cell line
2418 http://bard.broadinstitute.org/bard#2418 A431 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> A431
1931 http://www.bard.nih.gov/ontology/bard#BARD_0001812 A498 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> A498
A498 Renal Cancer cell line
713 http://www.bard.nih.gov/ontology/bard#BARD_0000718 A549 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> A549
Open Biomedical Ontologies - CLO : http://purl.obolibrary.org/obo/CLO_0001601
2419 http://bard.broadinstitute.org/bard#2419 A549/ATCC BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> A549/ATCC
1888 http://www.bard.nih.gov/ontology/bard#BARD_0001769 A673 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> A673
A673 cell line
714 http://www.bard.nih.gov/ontology/bard#BARD_0000719 ACHN BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> ACHN
Open Biomedical Ontologies - CLO : http://purl.obolibrary.org/obo/CLO_0001654
1928 http://www.bard.nih.gov/ontology/bard#BARD_0001809 ADR-RES BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> ADR-RES
ADR-RES Ovarian Cancer cell line
715 http://www.bard.nih.gov/ontology/bard#BARD_0000720 AML12 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> AML12
Open Biomedical Ontologies - CLO : http://purl.obolibrary.org/obo/CLO_0001716
716 http://www.bard.nih.gov/ontology/bard#BARD_0000721 BA/F3 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> BA/F3
Open Biomedical Ontologies - CLO : http://purl.obolibrary.org/obo/CLO_0001842
2391 http://bard.broadinstitute.org/bard#2391 BESM BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> BESM
Bovine Embryo Skeletal Muscle
1887 http://www.bard.nih.gov/ontology/bard#BARD_0001768 BHK-21 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> BHK-21
Baby Hamster Kidney cell line
1578 http://www.bard.nih.gov/ontology/bard#BARD_0001462 BJ BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> BJ
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002670
1579 http://www.bard.nih.gov/ontology/bard#BARD_0001463 BSC-1 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> BSC-1
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002678
1580 http://www.bard.nih.gov/ontology/bard#BARD_0001464 BSR BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> BSR
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002718
717 http://www.bard.nih.gov/ontology/bard#BARD_0000722 BT-549 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> BT-549
Open Biomedical Ontologies - CLO : http://purl.obolibrary.org/obo/CLO_0002044
1868 http://www.bard.nih.gov/ontology/bard#BARD_0001752 BW5147 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> BW5147
BW5147 Murine cells
718 http://www.bard.nih.gov/ontology/bard#BARD_0000723 C-33A BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> C-33A
Open Biomedical Ontologies - CLO : http://purl.obolibrary.org/obo/CLO_0002115
1581 http://www.bard.nih.gov/ontology/bard#BARD_0001465 C2C12 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> C2C12
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002708
1582 http://www.bard.nih.gov/ontology/bard#BARD_0001466 C6 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> C6
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002703
719 http://www.bard.nih.gov/ontology/bard#BARD_0000724 CAKI-1 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> CAKI-1
Open Biomedical Ontologies - CLO : http://purl.obolibrary.org/obo/CLO_0002175
2427 http://bard.broadinstitute.org/bard#2427 CALU-1 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> CALU-1
2428 http://bard.broadinstitute.org/bard#2428 CCD-19LU BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> CCD-19LU
720 http://www.bard.nih.gov/ontology/bard#BARD_0000725 CCRF-CEM BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> CCRF-CEM
Open Biomedical Ontologies - CLO : http://purl.obolibrary.org/obo/CLO_0002334
1941 http://www.bard.nih.gov/ontology/bard#BARD_0001822 CEM-SS BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> CEM-SS
CEM-SS cell line
1886 http://www.bard.nih.gov/ontology/bard#BARD_0001767 CEM21 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> CEM21
CEM 21 T-Cell line
2429 http://bard.broadinstitute.org/bard#2429 CHA-59 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> CHA-59
2317 http://bard.broadinstitute.org/bard#2317 CHL-1 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> CHL-1
ATCC CRL-9446, this is a derivative of the RPMI 7932 human melanoma cell line (Bowes).
721 http://www.bard.nih.gov/ontology/bard#BARD_0000726 CHO BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> CHO
Open Biomedical Ontologies - CLO : http://purl.obolibrary.org/obo/CLO_0002421
722 http://www.bard.nih.gov/ontology/bard#BARD_0000727 CHO-K1 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> CHO-K1
Open Biomedical Ontologies - CLO : http://purl.obolibrary.org/obo/CLO_0002462
2430 http://bard.broadinstitute.org/bard#2430 CHO/159-1 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> CHO/159-1
2431 http://bard.broadinstitute.org/bard#2431 COLO 205 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> COLO 205
2432 http://bard.broadinstitute.org/bard#2432 COLO 741 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> COLO 741
2433 http://bard.broadinstitute.org/bard#2433 COLO 746 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> COLO 746
723 http://www.bard.nih.gov/ontology/bard#BARD_0000728 COLO205 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> COLO205
Open Biomedical Ontologies - CLO : http://purl.obolibrary.org/obo/CLO_0002544
724 http://www.bard.nih.gov/ontology/bard#BARD_0000729 COS-7 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> COS-7
Open Biomedical Ontologies - CLO : http://purl.obolibrary.org/obo/CLO_0002597
1622 http://www.bard.nih.gov/ontology/bard#BARD_0001506 CRL-1963 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> CRL-1963
BioAssay Ontology v2 : http://www.bioassayontology.org/bao2.0_development#BAO_0000743
1938 http://www.bard.nih.gov/ontology/bard#BARD_0001819 CRL-2307 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> CRL-2307
Wild type primary dermal human fibroblasts
1583 http://www.bard.nih.gov/ontology/bard#BARD_0001467 CSM14.1 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> CSM14.1
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002717
2434 http://bard.broadinstitute.org/bard#2434 CXF 264L BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> CXF 264L
2012 http://bard.broadinstitute.org/bard#2012 Caco-2 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> Caco-2
human epithelial colorectal adenocarcinoma cell line
2398 http://bard.broadinstitute.org/bard#2398 Chem-1 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> Chem-1
EMD Millipore, rat
1999 http://bard.broadinstitute.org/bard#1999 Cos-1 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> Cos-1
Fibroblast-like cell line
1885 http://www.bard.nih.gov/ontology/bard#BARD_0001766 D54 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> D54
D54 T-Cell line
2435 http://bard.broadinstitute.org/bard#2435 DB BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> DB
2284 http://bard.broadinstitute.org/bard#2284 DHL10 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> DHL10
Round lymphoid cell line
725 http://www.bard.nih.gov/ontology/bard#BARD_0000730 DLD-1 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> DLD-1
Open Biomedical Ontologies - CLO : http://purl.obolibrary.org/obo/CLO_0002785
2436 http://bard.broadinstitute.org/bard#2436 DMS 114 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> DMS 114
2437 http://bard.broadinstitute.org/bard#2437 DMS 273 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> DMS 273
2438 http://bard.broadinstitute.org/bard#2438 DU-145 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> DU-145
726 http://www.bard.nih.gov/ontology/bard#BARD_0000731 DU145 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> DU145
Open Biomedical Ontologies - CLO : http://purl.obolibrary.org/obo/CLO_0002840
1841 http://bard.broadinstitute.org/bard#1841 Do Not Use #1841 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> Do Not Use #1841
1902 http://www.bard.nih.gov/ontology/bard#BARD_0001783 EKVX BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> EKVX
EKVX Lung Cancer cell line
2285 http://bard.broadinstitute.org/bard#2285 EL-4 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> EL-4
Mouse lymphona cell line
2371 http://bard.broadinstitute.org/bard#2371 EL-4 murine T lymphocyte BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> EL-4 murine T lymphocyte
EL-4 murine T lymphocyte cell line
2439 http://bard.broadinstitute.org/bard#2439 ES-2 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> ES-2
1741 http://www.bard.nih.gov/ontology/bard#BARD_0001565 F-2111 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> F-2111
1742 http://www.bard.nih.gov/ontology/bard#BARD_0001566 F-2112 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> F-2112
1740 http://www.bard.nih.gov/ontology/bard#BARD_0001564 F-87 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> F-87
1422 http://www.bard.nih.gov/ontology/bard#BARD_0001432 Fa2N-4 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> Fa2N-4
from UNM min Assay annnotaion xls
727 http://www.bard.nih.gov/ontology/bard#BARD_0000732 H-4-II-E BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> H-4-II-E
Open Biomedical Ontologies - CLO : http://purl.obolibrary.org/obo/CLO_0003603
2440 http://bard.broadinstitute.org/bard#2440 H1299p53RE29 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> H1299p53RE29
1905 http://www.bard.nih.gov/ontology/bard#BARD_0001786 H226 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> H226
H226 Lung Cancer cell line
1906 http://www.bard.nih.gov/ontology/bard#BARD_0001787 H322M BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> H322M
H322M Lung Cancer cell line
1584 http://www.bard.nih.gov/ontology/bard#BARD_0001468 H4 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> H4
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002713
1907 http://www.bard.nih.gov/ontology/bard#BARD_0001788 H460 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> H460
H460 Lung Cancer cell line
1908 http://www.bard.nih.gov/ontology/bard#BARD_0001789 H522 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> H522
H522 Lung Cancer cell line
728 http://www.bard.nih.gov/ontology/bard#BARD_0000733 H69AR BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> H69AR
Open Biomedical Ontologies - CLO : http://purl.obolibrary.org/obo/CLO_0003610
2292 http://bard.broadinstitute.org/bard#2292 H9c2 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> H9c2
H9c2 embryonic myocardium cells
1909 http://www.bard.nih.gov/ontology/bard#BARD_0001790 HCC-2998 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> HCC-2998
HCC=2998 Colon Cancer cell line
2441 http://bard.broadinstitute.org/bard#2441 HCT-116 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> HCT-116
2442 http://bard.broadinstitute.org/bard#2442 HCT-116/CMV-1 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> HCT-116/CMV-1
2443 http://bard.broadinstitute.org/bard#2443 HCT-116/CMV-2 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> HCT-116/CMV-2
2444 http://bard.broadinstitute.org/bard#2444 HCT-116/E6-1 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> HCT-116/E6-1
2445 http://bard.broadinstitute.org/bard#2445 HCT-116/E6-2 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> HCT-116/E6-2
2446 http://bard.broadinstitute.org/bard#2446 HCT-116/P BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> HCT-116/P
2447 http://bard.broadinstitute.org/bard#2447 HCT-116/P21/A BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> HCT-116/P21/A
2448 http://bard.broadinstitute.org/bard#2448 HCT-116/P21/B BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> HCT-116/P21/B
2449 http://bard.broadinstitute.org/bard#2449 HCT-116/P21/C BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> HCT-116/P21/C
2450 http://bard.broadinstitute.org/bard#2450 HCT-116/PV BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> HCT-116/PV
1910 http://www.bard.nih.gov/ontology/bard#BARD_0001791 HCT-15 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> HCT-15
HCT-15 Colon Cancer cell line
729 http://www.bard.nih.gov/ontology/bard#BARD_0000734 HCT116 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> HCT116
Open Biomedical Ontologies - CLO : http://purl.obolibrary.org/obo/CLO_0003665
1585 http://www.bard.nih.gov/ontology/bard#BARD_0001469 HEC-1-A BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> HEC-1-A
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002709
730 http://www.bard.nih.gov/ontology/bard#BARD_0000735 HEL BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> HEL
Open Biomedical Ontologies - CLO : http://purl.obolibrary.org/obo/CLO_0003679
733 http://www.bard.nih.gov/ontology/bard#BARD_0000738 HL-60 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> HL-60
Open Biomedical Ontologies - CLO : http://purl.obolibrary.org/obo/CLO_0003775
2451 http://bard.broadinstitute.org/bard#2451 HL-60(TB) BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> HL-60(TB)
2313 http://bard.broadinstitute.org/bard#2313 HMC 1-8 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> HMC 1-8
HMC-1-8 is a target cell of the T-cell killer clone T(cHMC-1) derived from breast cancer established from pleural effusion.
1870 http://www.bard.nih.gov/ontology/bard#BARD_0001754 HMLE BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> HMLE
Human Mammary Epithelial Cells
2452 http://bard.broadinstitute.org/bard#2452 HOP-18 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> HOP-18
1903 http://www.bard.nih.gov/ontology/bard#BARD_0001784 HOP-62 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> HOP-62
HOP-62 Lung Cancer cell line
1904 http://www.bard.nih.gov/ontology/bard#BARD_0001785 HOP-92 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> HOP-92
HOP-92 Lung Cancer cell line
2453 http://bard.broadinstitute.org/bard#2453 HS 578T BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> HS 578T
2454 http://bard.broadinstitute.org/bard#2454 HT BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> HT
735 http://www.bard.nih.gov/ontology/bard#BARD_0000740 HT-1080 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> HT-1080
Open Biomedical Ontologies - CLO : http://purl.obolibrary.org/obo/CLO_0004276
736 http://www.bard.nih.gov/ontology/bard#BARD_0000741 HT-29 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> HT-29
Open Biomedical Ontologies - CLO : http://purl.obolibrary.org/obo/CLO_0004283
2455 http://bard.broadinstitute.org/bard#2455 HT29 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> HT29
2456 http://bard.broadinstitute.org/bard#2456 HT29p53RE22 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> HT29p53RE22
737 http://www.bard.nih.gov/ontology/bard#BARD_0000742 HUV-EC-C BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> HUV-EC-C
Open Biomedical Ontologies - CLO : http://purl.obolibrary.org/obo/CLO_0004307
2402 http://bard.broadinstitute.org/bard#2402 Hamster primary cells BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> Hamster primary cells
hamster
731 http://www.bard.nih.gov/ontology/bard#BARD_0000736 HeLa BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> HeLa
Open Biomedical Ontologies - CLO : http://purl.obolibrary.org/obo/CLO_0003684
732 http://www.bard.nih.gov/ontology/bard#BARD_0000737 HepG2 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> HepG2
Open Biomedical Ontologies - CLO : http://purl.obolibrary.org/obo/CLO_0003704
734 http://www.bard.nih.gov/ontology/bard#BARD_0000739 Hs578T BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> Hs578T
Open Biomedical Ontologies - CLO : http://purl.obolibrary.org/obo/CLO_0004009
1859 http://www.bard.nih.gov/ontology/bard#BARD_0001744 Huh7 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> Huh7
Huh7 cell line
738 http://www.bard.nih.gov/ontology/bard#BARD_0000743 IEC-6 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> IEC-6
Open Biomedical Ontologies - CLO : http://purl.obolibrary.org/obo/CLO_0004342
1923 http://www.bard.nih.gov/ontology/bard#BARD_0001804 IGROV1 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> IGROV1
IGROV1 Ovarian Cancer cell line
2401 http://bard.broadinstitute.org/bard#2401 IMR-32 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> IMR-32
brain; derived from metastatic site: abdominal mass
2288 http://bard.broadinstitute.org/bard#2288 IMR-90 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> IMR-90
Human Caucasian fetal lung fibroblast cell line
2372 http://bard.broadinstitute.org/bard#2372 INS-1E BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> INS-1E
Rat insulinoma beta cell pancreatic cell line
1897 http://www.bard.nih.gov/ontology/bard#BARD_0001778 IgMXP3 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> IgMXP3
ABCG2 Expressing cell line
2392 http://bard.broadinstitute.org/bard#2392 J774 macrophages BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> J774 macrophages
J774 macrophages
2457 http://bard.broadinstitute.org/bard#2457 JCA-1 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> JCA-1
739 http://www.bard.nih.gov/ontology/bard#BARD_0000744 Jurkat BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> Jurkat
Open Biomedical Ontologies - CLO : http://purl.obolibrary.org/obo/CLO_0007043
2458 http://bard.broadinstitute.org/bard#2458 K-562 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> K-562
2283 http://bard.broadinstitute.org/bard#2283 K562 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> K562
Erythroleukemia cell line
1911 http://www.bard.nih.gov/ontology/bard#BARD_0001792 KM12 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> KM12
KM12 Colon Cancer cell line
2459 http://bard.broadinstitute.org/bard#2459 KM20L2 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> KM20L2
1586 http://www.bard.nih.gov/ontology/bard#BARD_0001470 KOPN-8 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> KOPN-8
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002715
1891 http://www.bard.nih.gov/ontology/bard#BARD_0001772 L-6 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> L-6
L-6 Rat skeletal myoblast cell line
1878 http://www.bard.nih.gov/ontology/bard#BARD_0001760 LCL BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> LCL
EBV-transfomed lymphoblastoid cells
2380 http://bard.broadinstitute.org/bard#2380 LLC-MK2 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> LLC-MK2
rhesus monkey kidney epithelial cell line
1919 http://www.bard.nih.gov/ontology/bard#BARD_0001800 LOX IMVI BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> LOX IMVI
LOX IMVI Melanoma cell line
2460 http://bard.broadinstitute.org/bard#2460 LXFL 529 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> LXFL 529
2461 http://bard.broadinstitute.org/bard#2461 LXFS 650L BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> LXFS 650L
1587 http://www.bard.nih.gov/ontology/bard#BARD_0001471 LYMP1-002 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> LYMP1-002
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002702
1588 http://www.bard.nih.gov/ontology/bard#BARD_0001472 LYMP1-003 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> LYMP1-003
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002701
1589 http://www.bard.nih.gov/ontology/bard#BARD_0001473 LYMP2-001 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> LYMP2-001
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002674
1590 http://www.bard.nih.gov/ontology/bard#BARD_0001474 LYMP2-002 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> LYMP2-002
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002688
1591 http://www.bard.nih.gov/ontology/bard#BARD_0001475 LYMP2-003 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> LYMP2-003
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002700
1592 http://www.bard.nih.gov/ontology/bard#BARD_0001476 LYMP2-004 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> LYMP2-004
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002699
1593 http://www.bard.nih.gov/ontology/bard#BARD_0001477 LYMP2-005 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> LYMP2-005
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002698
1594 http://www.bard.nih.gov/ontology/bard#BARD_0001478 LYMP2-006 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> LYMP2-006
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002687
1595 http://www.bard.nih.gov/ontology/bard#BARD_0001479 LYMP2-007 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> LYMP2-007
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002686
1596 http://www.bard.nih.gov/ontology/bard#BARD_0001480 LYMP2-008 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> LYMP2-008
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002704
1597 http://www.bard.nih.gov/ontology/bard#BARD_0001481 LYMP2-009 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> LYMP2-009
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002697
1598 http://www.bard.nih.gov/ontology/bard#BARD_0001482 LYMP2-010 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> LYMP2-010
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002673
1599 http://www.bard.nih.gov/ontology/bard#BARD_0001483 LYMP2-011 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> LYMP2-011
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002696
1600 http://www.bard.nih.gov/ontology/bard#BARD_0001484 LYMP2-012 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> LYMP2-012
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002675
1601 http://www.bard.nih.gov/ontology/bard#BARD_0001485 LYMP2-013 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> LYMP2-013
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002695
1602 http://www.bard.nih.gov/ontology/bard#BARD_0001486 LYMP2-014 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> LYMP2-014
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002676
1603 http://www.bard.nih.gov/ontology/bard#BARD_0001487 LYMP2-015 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> LYMP2-015
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002706
1604 http://www.bard.nih.gov/ontology/bard#BARD_0001488 LYMP2-016 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> LYMP2-016
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002677
1605 http://www.bard.nih.gov/ontology/bard#BARD_0001489 LYMP2-017 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> LYMP2-017
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002693
1606 http://www.bard.nih.gov/ontology/bard#BARD_0001490 LYMP2-018 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> LYMP2-018
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002679
1607 http://www.bard.nih.gov/ontology/bard#BARD_0001491 LYMP2-019 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> LYMP2-019
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002692
1608 http://www.bard.nih.gov/ontology/bard#BARD_0001492 LYMP2-020 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> LYMP2-020
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002680
1609 http://www.bard.nih.gov/ontology/bard#BARD_0001493 LYMP2-021 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> LYMP2-021
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002691
1610 http://www.bard.nih.gov/ontology/bard#BARD_0001494 LYMP2-022 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> LYMP2-022
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002681
1611 http://www.bard.nih.gov/ontology/bard#BARD_0001495 LYMP2-023 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> LYMP2-023
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002690
1612 http://www.bard.nih.gov/ontology/bard#BARD_0001496 LYMP2-024 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> LYMP2-024
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002682
1613 http://www.bard.nih.gov/ontology/bard#BARD_0001497 LYMP2-025 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> LYMP2-025
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002689
1614 http://www.bard.nih.gov/ontology/bard#BARD_0001498 LYMP2-026 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> LYMP2-026
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002684
1920 http://www.bard.nih.gov/ontology/bard#BARD_0001801 M14 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> M14
M14 Melanoma cell line
2462 http://bard.broadinstitute.org/bard#2462 M19-MEL BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> M19-MEL
2463 http://bard.broadinstitute.org/bard#2463 MALME-3M BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> MALME-3M
1874 http://www.bard.nih.gov/ontology/bard#BARD_0001757 MALME3 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> MALME3
MALME3 Cells
2465 http://bard.broadinstitute.org/bard#2465 MAXF 401 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> MAXF 401
740 http://www.bard.nih.gov/ontology/bard#BARD_0000745 MCF7 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> MCF7
Open Biomedical Ontologies - CLO : http://purl.obolibrary.org/obo/CLO_0007606
2467 http://bard.broadinstitute.org/bard#2467 MCF7-E6 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> MCF7-E6
2466 http://bard.broadinstitute.org/bard#2466 MCF7/ATCC BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> MCF7/ATCC
741 http://www.bard.nih.gov/ontology/bard#BARD_0000746 MDA-MB-231 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> MDA-MB-231
Open Biomedical Ontologies - CLO : http://purl.obolibrary.org/obo/CLO_0007634
2468 http://bard.broadinstitute.org/bard#2468 MDA-MB-231/ATCC BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> MDA-MB-231/ATCC
1847 http://www.bard.nih.gov/ontology/bard#BARD_0001734 MDA-MB-435 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> MDA-MB-435
2469 http://bard.broadinstitute.org/bard#2469 MDA-MB-435S BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> MDA-MB-435S
2470 http://bard.broadinstitute.org/bard#2470 MDA-MB-468 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> MDA-MB-468
2471 http://bard.broadinstitute.org/bard#2471 MDA-N BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> MDA-N
742 http://www.bard.nih.gov/ontology/bard#BARD_0000747 MDCK BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> MDCK
Open Biomedical Ontologies - CLO : http://purl.obolibrary.org/obo/CLO_0007646
2314 http://bard.broadinstitute.org/bard#2314 MEF-1 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> MEF-1
Mouse embryonic fibroblasts, ATCC CRL-2214. The MEF-1 cell line was initiated in 1993 by transfection of mouse embryo fibroblasts with an SV40 coding plasmid.
2472 http://bard.broadinstitute.org/bard#2472 MEXF 514L BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> MEXF 514L
1615 http://www.bard.nih.gov/ontology/bard#BARD_0001499 ML-2 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> ML-2
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002714
2473 http://bard.broadinstitute.org/bard#2473 MLI-019 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> MLI-019
2474 http://bard.broadinstitute.org/bard#2474 MLI-045 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> MLI-045
2475 http://bard.broadinstitute.org/bard#2475 MLI-059 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> MLI-059
2476 http://bard.broadinstitute.org/bard#2476 MLI-076 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> MLI-076
1899 http://www.bard.nih.gov/ontology/bard#BARD_0001780 MOLT-4 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> MOLT-4
MOLT-4 Leukemia cell line
743 http://www.bard.nih.gov/ontology/bard#BARD_0000748 MRC-5 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> MRC-5
Open Biomedical Ontologies - CLO : http://purl.obolibrary.org/obo/CLO_0007865
1616 http://www.bard.nih.gov/ontology/bard#BARD_0001577 MSC80 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> MSC80
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002705
1617 http://www.bard.nih.gov/ontology/bard#BARD_0001501 MV-4-11 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> MV-4-11
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002685
2464 http://bard.broadinstitute.org/bard#2464 Mar-Bel BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> Mar-Bel
2315 http://bard.broadinstitute.org/bard#2315 MeWo BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> MeWo
The MeWo cell line was initiated by Y. Kodera and M. Bean in 1974 from lymph node tissue. Skin; Derived from metastatic site: lymph node
2316 http://bard.broadinstitute.org/bard#2316 Mel501 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> Mel501
Human melanoma cell line.
1618 http://www.bard.nih.gov/ontology/bard#BARD_0001502 N2a BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> N2a
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002711
2477 http://bard.broadinstitute.org/bard#2477 NB4 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> NB4
2479 http://bard.broadinstitute.org/bard#2479 NCI-H226 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> NCI-H226
2480 http://bard.broadinstitute.org/bard#2480 NCI-H23 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> NCI-H23
2481 http://bard.broadinstitute.org/bard#2481 NCI-H322M BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> NCI-H322M
2482 http://bard.broadinstitute.org/bard#2482 NCI-H460 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> NCI-H460
2483 http://bard.broadinstitute.org/bard#2483 NCI-H522 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> NCI-H522
2478 http://bard.broadinstitute.org/bard#2478 NCI/ADR-RES BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> NCI/ADR-RES
2484 http://bard.broadinstitute.org/bard#2484 ND-1 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> ND-1
744 http://www.bard.nih.gov/ontology/bard#BARD_0000749 NIH-3T3 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> NIH-3T3
Open Biomedical Ontologies - CLO : http://purl.obolibrary.org/obo/CLO_0008172
2485 http://bard.broadinstitute.org/bard#2485 NYH BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> NYH
2486 http://bard.broadinstitute.org/bard#2486 NYH/ICRF-187-1 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> NYH/ICRF-187-1
1867 http://www.bard.nih.gov/ontology/bard#BARD_0001751 Neuro-2A BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> Neuro-2A
Neuro-2A murine neuroblastoma cells
1619 http://www.bard.nih.gov/ontology/bard#BARD_0001503 OCI-Ly3 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> OCI-Ly3
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002707
2487 http://bard.broadinstitute.org/bard#2487 OHS BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> OHS
1924 http://www.bard.nih.gov/ontology/bard#BARD_0001805 OVCAR-3 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> OVCAR-3
OVCAR-3 Ovarian Cancer cell line
1925 http://www.bard.nih.gov/ontology/bard#BARD_0001806 OVCAR-4 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> OVCAR-4
OVCAR-3 Ovarian Cancer cell line
1926 http://www.bard.nih.gov/ontology/bard#BARD_0001807 OVCAR-5 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> OVCAR-5
OVCAR-3 Ovarian Cancer cell line
1927 http://www.bard.nih.gov/ontology/bard#BARD_0001808 OVCAR-8 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> OVCAR-8
OVCAR-3 Ovarian Cancer cell line
2488 http://bard.broadinstitute.org/bard#2488 P388 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> P388
2489 http://bard.broadinstitute.org/bard#2489 P388/ADR BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> P388/ADR
1935 http://www.bard.nih.gov/ontology/bard#BARD_0001816 PC-3 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> PC-3
PC-3 Ovarian Cancer cell line
2287 http://bard.broadinstitute.org/bard#2287 PC3M BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> PC3M
Human prostate cancer cell line
1620 http://www.bard.nih.gov/ontology/bard#BARD_0001504 PPC-1 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> PPC-1
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002716
2406 http://bard.broadinstitute.org/bard#2406 Primary human melanocytes BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> Primary human melanocytes
Primary melanocytes cultured from human source
1621 http://www.bard.nih.gov/ontology/bard#BARD_0001505 RBL-2H3 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> RBL-2H3
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002694
1940 http://www.bard.nih.gov/ontology/bard#BARD_0001821 RD BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> RD
Rhabdomycosarcoma cell line
2490 http://bard.broadinstitute.org/bard#2490 RH30 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> RH30
2491 http://bard.broadinstitute.org/bard#2491 RKO Waf1 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> RKO Waf1
2492 http://bard.broadinstitute.org/bard#2492 RKOp53RE1 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> RKOp53RE1
2493 http://bard.broadinstitute.org/bard#2493 RL BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> RL
2494 http://bard.broadinstitute.org/bard#2494 RPMI-7951 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> RPMI-7951
1900 http://www.bard.nih.gov/ontology/bard#BARD_0001781 RPMI-8226 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> RPMI-8226
RPMI-8226 Leukemia cell line
1932 http://www.bard.nih.gov/ontology/bard#BARD_0001813 RXF 393 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> RXF 393
RXF 393 Renal Cancer cell line
2495 http://bard.broadinstitute.org/bard#2495 RXF 486L BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> RXF 486L
2496 http://bard.broadinstitute.org/bard#2496 RXF-631 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> RXF-631
2295 http://bard.broadinstitute.org/bard#2295 Raji BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> Raji
2397 http://bard.broadinstitute.org/bard#2397 Rat primary cells BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> Rat primary cells
Rat Primary Cells
1793 http://www.bard.nih.gov/ontology/bard#BARD_0001689 S1P1 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> S1P1
1794 http://www.bard.nih.gov/ontology/bard#BARD_0001690 S1P3 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> S1P3
1756 http://www.bard.nih.gov/ontology/bard#BARD_0001578 S2 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> S2
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000743
2282 http://bard.broadinstitute.org/bard#2282 SCC-12F BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> SCC-12F
Human squamous cell line
1838 http://www.bard.nih.gov/ontology/bard#BARD_0001725 SDS-PAGE BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> SDS-PAGE
1913 http://www.bard.nih.gov/ontology/bard#BARD_0001794 SF-268 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> SF-268
SF-268 CNS Cancer cell line
1914 http://www.bard.nih.gov/ontology/bard#BARD_0001795 SF-295 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> SF-295
SF-295 CNS Cancer cell line
1915 http://www.bard.nih.gov/ontology/bard#BARD_0001796 SF-539 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> SF-539
SF-539 CNS Cancer cell line
2497 http://bard.broadinstitute.org/bard#2497 SF-767 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> SF-767
745 http://www.bard.nih.gov/ontology/bard#BARD_0000750 SH-SY5Y BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> SH-SY5Y
Open Biomedical Ontologies - CLO : http://purl.obolibrary.org/obo/CLO_0009015
1623 http://www.bard.nih.gov/ontology/bard#BARD_0001507 SK-BR-3 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> SK-BR-3
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002712
2498 http://bard.broadinstitute.org/bard#2498 SK-MEL-2 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> SK-MEL-2
2499 http://bard.broadinstitute.org/bard#2499 SK-MEL-28 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> SK-MEL-28
1873 http://www.bard.nih.gov/ontology/bard#BARD_0001756 SK-MEL-5 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> SK-MEL-5
SK-MEL-5 Cells
746 http://www.bard.nih.gov/ontology/bard#BARD_0000751 SK-N-SH BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> SK-N-SH
Open Biomedical Ontologies - CLO : http://purl.obolibrary.org/obo/CLO_0009059
1929 http://www.bard.nih.gov/ontology/bard#BARD_0001810 SK-OV-3 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> SK-OV-3
SK-OV-3 Ovarian Cancer cell line
2500 http://bard.broadinstitute.org/bard#2500 SMS-KCNR BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> SMS-KCNR
2501 http://bard.broadinstitute.org/bard#2501 SN12C BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> SN12C
2502 http://bard.broadinstitute.org/bard#2502 SN12K1 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> SN12K1
1916 http://www.bard.nih.gov/ontology/bard#BARD_0001797 SNB-19 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> SNB-19
SNB-19 CNS Cancer cell line
1917 http://www.bard.nih.gov/ontology/bard#BARD_0001798 SNB-75 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> SNB-75
SNB-75 CNS Cancer cell line
2503 http://bard.broadinstitute.org/bard#2503 SNB-78 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> SNB-78
1901 http://www.bard.nih.gov/ontology/bard#BARD_0001782 SR BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> SR
SR Leukemia cell line
1877 http://www.bard.nih.gov/ontology/bard#BARD_0001759 SUM159 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> SUM159
SUM159 cell line
1882 http://www.bard.nih.gov/ontology/bard#BARD_0001764 SV40 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> SV40
SV40 large T antigen-transformed osteoblast cell line
2504 http://bard.broadinstitute.org/bard#2504 SW 1088 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> SW 1088
2505 http://bard.broadinstitute.org/bard#2505 SW 1783 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> SW 1783
2506 http://bard.broadinstitute.org/bard#2506 SW-156 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> SW-156
2507 http://bard.broadinstitute.org/bard#2507 SW-1573 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> SW-1573
2508 http://bard.broadinstitute.org/bard#2508 SW-620 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> SW-620
1866 http://www.bard.nih.gov/ontology/bard#BARD_0001750 SW620 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> SW620
SW620 Human Colorectal Carcinoma cell line
2386 http://bard.broadinstitute.org/bard#2386 Sf9 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> Sf9
a clonal isolate of Spodoptera frugiperda Sf21 cells (IPLB-SF21-AE), are commonly used for recombinant protein production using baculovirus. They were originally established from ovarian tissue. They can be grown in the absence of serum, and can be cultured attached or in suspension.
1937 http://www.bard.nih.gov/ontology/bard#BARD_0001818 T-47D BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> T-47D
T-47D Breast Cancer cell line
2509 http://bard.broadinstitute.org/bard#2509 T47D ERE4 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> T47D ERE4
2510 http://bard.broadinstitute.org/bard#2510 T47D FOS1 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> T47D FOS1
2511 http://bard.broadinstitute.org/bard#2511 T47D NFkB15 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> T47D NFkB15
2512 http://bard.broadinstitute.org/bard#2512 TE85 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> TE85
747 http://www.bard.nih.gov/ontology/bard#BARD_0000752 THP-1 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> THP-1
Open Biomedical Ontologies - CLO : http://purl.obolibrary.org/obo/CLO_0009348
1933 http://www.bard.nih.gov/ontology/bard#BARD_0001814 TK-10 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> TK-10
TK-10 Renal Cancer cell line
2513 http://bard.broadinstitute.org/bard#2513 TK-164 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> TK-164
2514 http://bard.broadinstitute.org/bard#2514 TSU-PRI BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> TSU-PRI
748 http://www.bard.nih.gov/ontology/bard#BARD_0000753 U-2OS BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> U-2OS
Open Biomedical Ontologies - CLO : http://purl.obolibrary.org/obo/CLO_0009454
1918 http://www.bard.nih.gov/ontology/bard#BARD_0001799 U251 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> U251
U251 CNS Cancer cell line
1624 http://www.bard.nih.gov/ontology/bard#BARD_0001508 U3A BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> U3A
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002683
749 http://www.bard.nih.gov/ontology/bard#BARD_0000754 U937(CD59+) BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> U937(CD59+)
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000758
2515 http://bard.broadinstitute.org/bard#2515 UABLG22 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> UABLG22
2516 http://bard.broadinstitute.org/bard#2516 UABMEL3 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> UABMEL3
1921 http://www.bard.nih.gov/ontology/bard#BARD_0001802 UACC-257 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> UACC-257
UACC-257 Melanoma cell line
1922 http://www.bard.nih.gov/ontology/bard#BARD_0001803 UACC-62 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> UACC-62
UACC-62 Melanoma cell line
2517 http://bard.broadinstitute.org/bard#2517 UISO-BCA-1 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> UISO-BCA-1
1934 http://www.bard.nih.gov/ontology/bard#BARD_0001815 UO-31 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> UO-31
UO-31 Renal Cancer cell line
2518 http://bard.broadinstitute.org/bard#2518 UOK-57 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> UOK-57
2519 http://bard.broadinstitute.org/bard#2519 VDSO/CMV-8 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> VDSO/CMV-8
2520 http://bard.broadinstitute.org/bard#2520 VDSO/CMV-9 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> VDSO/CMV-9
2521 http://bard.broadinstitute.org/bard#2521 VDSO/E6-18 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> VDSO/E6-18
2522 http://bard.broadinstitute.org/bard#2522 VDSO/E6-19 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> VDSO/E6-19
2523 http://bard.broadinstitute.org/bard#2523 VDSO/P BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> VDSO/P
751 http://www.bard.nih.gov/ontology/bard#BARD_0000756 VERO76 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> VERO76
Open Biomedical Ontologies - CLO : http://purl.obolibrary.org/obo/CLO_0009526
750 http://www.bard.nih.gov/ontology/bard#BARD_0000755 Vero BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> Vero
Open Biomedical Ontologies - CLO : http://purl.obolibrary.org/obo/CLO_0009524
2524 http://bard.broadinstitute.org/bard#2524 WI-38 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> WI-38
1625 http://www.bard.nih.gov/ontology/bard#BARD_0001509 WRO BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> WRO
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002710
2525 http://bard.broadinstitute.org/bard#2525 XF 498 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> XF 498
2526 http://bard.broadinstitute.org/bard#2526 ZR-75-1 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> ZR-75-1
2527 http://bard.broadinstitute.org/bard#2527 ZR-75-30 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> ZR-75-30
2293 http://bard.broadinstitute.org/bard#2293 bY4741 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> bY4741
1889 http://www.bard.nih.gov/ontology/bard#BARD_0001770 mESC BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> mESC
mESC Cell Line
2389 http://bard.broadinstitute.org/bard#2389 mouse primary cells BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> cultured cell name> other cell name> mouse primary cells
primary cells derived from mice
1461 http://www.bard.nih.gov/ontology/bard#BARD_0001607 differentiated cultured cell BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured cell> differentiated cultured cell
An assay component type where a less specialized cell becomes more specialized in terms of cell type in a multicellular organism.
245 http://www.bard.nih.gov/ontology/bard#BARD_0000243 cultured organism BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured organism
Organism related to the target/meta-target of the bioassay; includes both prokaryotes and eukaryotes.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000551
1744 http://www.bard.nih.gov/ontology/bard#BARD_0001409 clinical isolate name BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured organism> clinical isolate name
378 http://www.bard.nih.gov/ontology/bard#BARD_0000376 cultured organism type BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured organism> cultured organism type
437 http://www.bard.nih.gov/ontology/bard#BARD_0000436 cellular organism BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured organism> cultured organism type> cellular organism
Includes all organisms which are made up of cells, e.g., bacteria, fungi, plants, and animals.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000658
487 http://www.bard.nih.gov/ontology/bard#BARD_0000487 bacterium BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured organism> cultured organism type> cellular organism> bacterium
Prokaryotic cell; bacteria lack membrane-bound organelles and are surrounded by a rigid cell wall. Though they are unicellular, they function by aggregating into multicellular colonies.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000364
488 http://www.bard.nih.gov/ontology/bard#BARD_0000488 eukaryote BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured organism> cultured organism type> cellular organism> eukaryote
Includes fungi, vertebrates, and invertebrates. Eukaryotic cells have a well-defined nucleus and membrane-enveloped organelles such as mitochondria, endoplasmic reticulum, Golgi apparatus, and lysosomes.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000509
514 http://www.bard.nih.gov/ontology/bard#BARD_0000514 fungus BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured organism> cultured organism type> cellular organism> eukaryote> fungus
Can be either unicellular or multicellular organisms that include yeasts, molds, and mushrooms. Several parasitic fungi exist that infect animals and plants, e.g., Aspergillus flavus, Claviceps purpurea, and Pneumocystis jirovecii.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000418
531 http://www.bard.nih.gov/ontology/bard#BARD_0000531 multicellular fungus BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured organism> cultured organism type> cellular organism> eukaryote> fungus> multicellular fungus
Fungus in which an individual organism consists of more than one cell. Most fungi are multicellular, and are made up of multicellular filaments known as hyphae. Several parasitic fungi exist, which infect animals and plants, such as Aspergillus flavus, Claviceps purpurea, and Pneumocystis jirovecii.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000553
532 http://www.bard.nih.gov/ontology/bard#BARD_0000532 unicellular fungus BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured organism> cultured organism type> cellular organism> eukaryote> fungus> unicellular fungus
Fungus in which an individual organisms consists of only one cell. This group includes the baker's yeast (Saccharomyces cerevisiae) and fission yeast (Schizosaccharomyces pombe).
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000233
515 http://www.bard.nih.gov/ontology/bard#BARD_0000515 metazoan BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured organism> cultured organism type> cellular organism> eukaryote> metazoan
Multicellular eukaryotic organisms that include those having a vertebral column (e.g., fish, frog, snake, bird, human) and those that do not (e.g., worm, fly, star fish, etc).
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000659
537 http://www.bard.nih.gov/ontology/bard#BARD_0000537 invertebrate BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured organism> cultured organism type> cellular organism> eukaryote> metazoan> invertebrate
Animals that lack a vertebral column. Many are used as model organisms in high throughput screening, including Drosophila, C. elegans, sea urchin, and others.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000511
538 http://www.bard.nih.gov/ontology/bard#BARD_0000538 vertebrate BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured organism> cultured organism type> cellular organism> eukaryote> metazoan> vertebrate
Animals that have a vertebral column. Many are used as model organisms, including the zebrafish, mouse, and rat.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000510
539 http://www.bard.nih.gov/ontology/bard#BARD_0000539 mammal BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured organism> cultured organism type> cellular organism> eukaryote> metazoan> vertebrate> mammal
Vertebrates with fur that bear live young. These include animal model organisms typically used in drug discovery for toxicity or pharmacology studies, such as mouse or rat.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000362
540 http://www.bard.nih.gov/ontology/bard#BARD_0000540 non-mammal BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured organism> cultured organism type> cellular organism> eukaryote> metazoan> vertebrate> non-mammal
Vertebrates that do not have fur and/or do not bear live young. These include animal model organisms such as the Danio rerio (zebrafish), Xenopus laevis, etc., which are often used to understand development.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000518
516 http://www.bard.nih.gov/ontology/bard#BARD_0000516 plant BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured organism> cultured organism type> cellular organism> eukaryote> plant
Organisms that derive their energy from photosynthesis, with the exception of parasitic plants. Plants include herbs, shrubs, trees, grasses, vines, etc.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000605
517 http://www.bard.nih.gov/ontology/bard#BARD_0000517 protozoan BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured organism> cultured organism type> cellular organism> eukaryote> protozoan
Single-celled organisms, some of which are parasitic. Examples include Plasmodium falciparum (causes malaria), Leishmania mexicana (causes leishmaniasis), and Trypanosoma brucei (causes sleeping sickness).
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000662
438 http://www.bard.nih.gov/ontology/bard#BARD_0000437 virus BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured organism> cultured organism type> virus
Small infectious agents that depend on the host cells to reproduce. Outside the host, they contain a protein coat called capsid that encloses their genetic material (either DNA or RNA). Viruses can infect bacteria, plants and animals and cause a number of diseases, e.g., smallpox, chickenpox, influenza, polio, rabies, etc.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000232
379 http://www.bard.nih.gov/ontology/bard#BARD_0000377 organism genetic modification BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured organism> organism genetic modification
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002057
2296 http://bard.broadinstitute.org/bard#2296 EuMyc BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured organism> organism genetic modification> EuMyc
463 http://www.bard.nih.gov/ontology/bard#BARD_0000462 engineered genotype BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured organism> organism genetic modification> engineered genotype
Open Biomedical Ontologies : http://purl.obolibrary.org/obo/ID_0000265
489 http://www.bard.nih.gov/ontology/bard#BARD_0000489 gene knockdown BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured organism> organism genetic modification> engineered genotype> gene knockdown
Technique by which the expression of one or more of an organism's genes is reduced, either through genetic modification (a change in the DNA of one of the organism's chromosomes) or by treatment with a reagent such as a short DNA or RNA oligonucleotide with a sequence complementary to either an mRNA transcript or a gene.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002433
520 http://www.bard.nih.gov/ontology/bard#BARD_0000520 gene knockdown by RNA interference BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured organism> organism genetic modification> engineered genotype> gene knockdown> gene knockdown by RNA interference
Method for introducing double-stranded RNA synthesized with a sequence complementary to a gene of interest into a cell or organism, where it is recognized as exogenous genetic material and activates the RNA interference (RNAi) pathway. Using this mechanism, researchers can cause a drastic decrease in the expression of a targeted gene.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002434
533 http://www.bard.nih.gov/ontology/bard#BARD_0000533 gene knockdown by dsRNA transfection BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured organism> organism genetic modification> engineered genotype> gene knockdown> gene knockdown by RNA interference> gene knockdown by dsRNA transfection
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002435
534 http://www.bard.nih.gov/ontology/bard#BARD_0000534 gene knockdown by lentiviral shRNA transduction BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured organism> organism genetic modification> engineered genotype> gene knockdown> gene knockdown by RNA interference> gene knockdown by lentiviral shRNA transduction
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002436
535 http://www.bard.nih.gov/ontology/bard#BARD_0000535 gene knockdown by shRNA transfection BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured organism> organism genetic modification> engineered genotype> gene knockdown> gene knockdown by RNA interference> gene knockdown by shRNA transfection
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002437
536 http://www.bard.nih.gov/ontology/bard#BARD_0000536 gene knockdown by siRNA transfection BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured organism> organism genetic modification> engineered genotype> gene knockdown> gene knockdown by RNA interference> gene knockdown by siRNA transfection
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002438
518 http://www.bard.nih.gov/ontology/bard#BARD_0000518 gene knockdown by antisense RNA BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured organism> organism genetic modification> engineered genotype> gene knockdown> gene knockdown by antisense RNA
Antisense RNA is a single-stranded RNA that is complementary to a messenger RNA (mRNA) strand transcribed within a cell. Antisense RNA may be introduced into a cell to inhibit translation of a complementary mRNA by base pairing to it and physically obstructing the translation machinery.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002439
519 http://www.bard.nih.gov/ontology/bard#BARD_0000519 gene knockdown by morpholino treatment BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured organism> organism genetic modification> engineered genotype> gene knockdown> gene knockdown by morpholino treatment
Method for reducing gene expression using morpholinos. Morpholino oligomers are an antisense technology used to block access of other molecules to specific sequences within nucleic acid. Morpholinos block small (~25 base) regions of the base-pairing surfaces of ribonucleic acid (RNA).
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002440
490 http://www.bard.nih.gov/ontology/bard#BARD_0000490 gene knockin BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured organism> organism genetic modification> engineered genotype> gene knockin
Technique by which a protein-coding cDNA sequence is inserted at a particular locus in an organism's chromosome. The difference between knock-in technology and transgenic technology is that a knock-in involves a gene inserted into a specific locus, and is a targeted insertion.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002432
491 http://www.bard.nih.gov/ontology/bard#BARD_0000491 gene knockout BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured organism> organism genetic modification> engineered genotype> gene knockout
Technique by which an organism is engineered to carry genes that have been made inoperative (have been knocked out of the organism).
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002441
492 http://www.bard.nih.gov/ontology/bard#BARD_0000492 gene overexpression BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured organism> organism genetic modification> engineered genotype> gene overexpression
Technique by which an organism or cell line is engineered to express increased levels of a gene.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002442
521 http://www.bard.nih.gov/ontology/bard#BARD_0000521 gene overexpression by stable transfection BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured organism> organism genetic modification> engineered genotype> gene overexpression> gene overexpression by stable transfection
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002443
522 http://www.bard.nih.gov/ontology/bard#BARD_0000522 gene overexpression by transient transfection BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured organism> organism genetic modification> engineered genotype> gene overexpression> gene overexpression by transient transfection
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002444
384 http://www.bard.nih.gov/ontology/bard#BARD_0000382 macromolecule BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured organism> organism genetic modification> macromolecule
Biology term that describes a protein, nucleic acid, lipid, or carbohydrate.
Open Biomedical Ontologies : http://purl.obolibrary.org/obo/ID_0000289
871 http://www.bard.nih.gov/ontology/bard#BARD_0001395 macromolecule description BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured organism> organism genetic modification> macromolecule> macromolecule description
A long name for a gene or protein from a trusted international source (e.g., Entrez, UniProt).
Open Biomedical Ontologies : http://purl.obolibrary.org/obo/ID_0000294
868 http://www.bard.nih.gov/ontology/bard#BARD_0001393 macromolecule identifier BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured organism> organism genetic modification> macromolecule> macromolecule identifier
An external database unique identifier, such as an accession number, for a gene or protein from a trusted international source (e.g., Entrez, UniProt).
Open Biomedical Ontologies : http://purl.obolibrary.org/obo/ID_0000290
1504 http://www.bard.nih.gov/ontology/bard#BARD_0001680 GO gene-product ID BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured organism> organism genetic modification> macromolecule> macromolecule identifier> GO gene-product ID
1795 http://www.bard.nih.gov/ontology/bard#BARD_0001691 NCBI accession number BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured organism> organism genetic modification> macromolecule> macromolecule identifier> NCBI accession number
1398 http://www.bard.nih.gov/ontology/bard#BARD_0001679 UniProt accession number BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured organism> organism genetic modification> macromolecule> macromolecule identifier> UniProt accession number
880 http://www.bard.nih.gov/ontology/bard#BARD_0000418 gene Entrez GI BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured organism> organism genetic modification> macromolecule> macromolecule identifier> gene Entrez GI
2414 http://bard.broadinstitute.org/bard#2414 nucleotide Entrez ID BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured organism> organism genetic modification> macromolecule> macromolecule identifier> nucleotide Entrez ID
The ID of a sequence in the entrez nucleotide database.
882 http://www.bard.nih.gov/ontology/bard#BARD_0001572 protein Entrez GI BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured organism> organism genetic modification> macromolecule> macromolecule identifier> protein Entrez GI
872 http://www.bard.nih.gov/ontology/bard#BARD_0001204 macromolecule name BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured organism> organism genetic modification> macromolecule> macromolecule name
A short symbol or name for a gene or protein from a trusted international source (e.g., Entrez, UniProt).
Open Biomedical Ontologies : http://purl.obolibrary.org/obo/ID_0000292
873 http://www.bard.nih.gov/ontology/bard#BARD_0001194 macromolecule sequence BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured organism> organism genetic modification> macromolecule> macromolecule sequence
870 http://www.bard.nih.gov/ontology/bard#BARD_0001193 macromolecule type BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured organism> organism genetic modification> macromolecule> macromolecule type
Open Biomedical Ontologies : http://purl.obolibrary.org/obo/ID_0000295
1316 http://www.bard.nih.gov/ontology/bard#BARD_0001583 antibody BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured organism> organism genetic modification> macromolecule> macromolecule type> antibody
1774 http://www.bard.nih.gov/ontology/bard#BARD_0001675 gene BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured organism> organism genetic modification> macromolecule> macromolecule type> gene
884 http://www.bard.nih.gov/ontology/bard#BARD_0001573 nucleotide BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured organism> organism genetic modification> macromolecule> macromolecule type> nucleotide
889 http://www.bard.nih.gov/ontology/bard#BARD_0001416 peptide BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured organism> organism genetic modification> macromolecule> macromolecule type> peptide
1785 http://www.bard.nih.gov/ontology/bard#BARD_0001676 protein BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured organism> organism genetic modification> macromolecule> macromolecule type> protein
A long polymer of amino acids joined together by peptide bonds (also, polypeptide). The sequence of the amino acids is defined by the genetic code.
1784 http://www.bard.nih.gov/ontology/bard#BARD_0001575 protein-DNA complex BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured organism> organism genetic modification> macromolecule> macromolecule type> protein-DNA complex
1314 http://www.bard.nih.gov/ontology/bard#BARD_0001581 protein-reporter complex BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured organism> organism genetic modification> macromolecule> macromolecule type> protein-reporter complex
2294 http://bard.broadinstitute.org/bard#2294 TORC1 BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured organism> organism genetic modification> macromolecule> macromolecule type> protein> TORC1
246 http://www.bard.nih.gov/ontology/bard#BARD_0000244 cultured tissue name BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured tissue name
Open Biomedical Ontologies - UBERON : http://purl.obolibrary.org/obo/UBERON_0000479
1508 http://www.bard.nih.gov/ontology/bard#BARD_0001448 brain BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured tissue name> brain
1505 http://www.bard.nih.gov/ontology/bard#BARD_0001445 heart BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured tissue name> heart
1506 http://www.bard.nih.gov/ontology/bard#BARD_0001446 kidney BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured tissue name> kidney
1507 http://www.bard.nih.gov/ontology/bard#BARD_0001447 liver BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured tissue name> liver
1660 http://www.bard.nih.gov/ontology/bard#BARD_0001539 mesencephalon BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured tissue name> mesencephalon
2403 http://bard.broadinstitute.org/bard#2403 pancreas BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured tissue name> pancreas
pancreas
1661 http://www.bard.nih.gov/ontology/bard#BARD_0001540 plasma BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured tissue name> plasma
1344 http://www.bard.nih.gov/ontology/bard#BARD_0001419 sperm BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured tissue name> sperm
2396 http://bard.broadinstitute.org/bard#2396 submaxillary gland BARD> assay protocol> assay component> assay component type> biological component> biological component type> cultured tissue name> submaxillary gland
submaxillary gland
247 http://www.bard.nih.gov/ontology/bard#BARD_0000245 deoxyribonucleic acid BARD> assay protocol> assay component> assay component type> biological component> biological component type> deoxyribonucleic acid
Open Biomedical Ontologies : http://purl.obolibrary.org/obo/ID_0000280
380 http://www.bard.nih.gov/ontology/bard#BARD_0000378 DNA construct sequence BARD> assay protocol> assay component> assay component type> biological component> biological component type> deoxyribonucleic acid> DNA construct sequence
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000300
381 http://www.bard.nih.gov/ontology/bard#BARD_0000379 DNA vector BARD> assay protocol> assay component> assay component type> biological component> biological component type> deoxyribonucleic acid> DNA vector
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002051
382 http://www.bard.nih.gov/ontology/bard#BARD_0000380 expressed component BARD> assay protocol> assay component> assay component type> biological component> biological component type> deoxyribonucleic acid> expressed component
448 http://www.bard.nih.gov/ontology/bard#BARD_0000447 expressed RNA BARD> assay protocol> assay component> assay component type> biological component> biological component type> deoxyribonucleic acid> expressed component> expressed RNA
499 http://www.bard.nih.gov/ontology/bard#BARD_0000499 RNA aptamer BARD> assay protocol> assay component> assay component type> biological component> biological component type> deoxyribonucleic acid> expressed component> expressed RNA> RNA aptamer
Aptamers are synthetic affinity probes. RNA aptamers are oligonucleotides that bind to a specific target molecule such as small molecules, proteins, nucleic acids, or cells. They are created by selecting them from a large random sequence pool, but natural aptamers also exist in riboswitches.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000496
496 http://www.bard.nih.gov/ontology/bard#BARD_0000496 esiRNA BARD> assay protocol> assay component> assay component type> biological component> biological component type> deoxyribonucleic acid> expressed component> expressed RNA> esiRNA
Endoribonuclease-prepared small interfering RNA (siRNA) pools comprised of a heterogeneous mixture of siRNAs that all target the same messenger RNA (mRNA) sequence.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000544
498 http://www.bard.nih.gov/ontology/bard#BARD_0000498 mRNA BARD> assay protocol> assay component> assay component type> biological component> biological component type> deoxyribonucleic acid> expressed component> expressed RNA> mRNA
messenger RNA (mRNA) is a genetic intermediate between DNA and protein that is transcribed from DNA by RNA polymerases and further processed by other enzymes. mRNA serves as the template to synthesize proteins on the ribosomes.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000274
497 http://www.bard.nih.gov/ontology/bard#BARD_0000497 miRNA BARD> assay protocol> assay component> assay component type> biological component> biological component type> deoxyribonucleic acid> expressed component> expressed RNA> miRNA
Short RNAs (~22 nucleotides long) known as microRNAs (miRNAs) that bind to complementary sequences in the three prime untranslated regions (3' UTRs) of target messenger RNA transcripts (mRNAs), usually resulting in gene silencing.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000322
500 http://www.bard.nih.gov/ontology/bard#BARD_0000500 shRNA BARD> assay protocol> assay component> assay component type> biological component> biological component type> deoxyribonucleic acid> expressed component> expressed RNA> shRNA
Short hairpin RNA (shRNA) can be used to achieve stable knockdown of gene expression. shRNA molecules are cloned into lentiviral or other vectors and expressed by a pol III type promoter.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000323
501 http://www.bard.nih.gov/ontology/bard#BARD_0000501 siRNA BARD> assay protocol> assay component> assay component type> biological component> biological component type> deoxyribonucleic acid> expressed component> expressed RNA> siRNA
In the RNAi pathway, the double stranded RNAs first get processed into 20-25 nucleotide small interfering RNAs (siRNAs) by an RNase-III-like enzyme called Dicer. The siRNAs enter into endoribonuclease-containing complexes known as RNA-induced silencing complexes (RISCs), and bring about the cleavage of the target mRNAs to achieve gene silencing.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000324
447 http://www.bard.nih.gov/ontology/bard#BARD_0000446 expressed protein BARD> assay protocol> assay component> assay component type> biological component> biological component type> deoxyribonucleic acid> expressed component> expressed protein
493 http://www.bard.nih.gov/ontology/bard#BARD_0000493 exogenous protein BARD> assay protocol> assay component> assay component type> biological component> biological component type> deoxyribonucleic acid> expressed component> expressed protein> exogenous protein
494 http://www.bard.nih.gov/ontology/bard#BARD_0000494 overexpressed protein BARD> assay protocol> assay component> assay component type> biological component> biological component type> deoxyribonucleic acid> expressed component> expressed protein> overexpressed protein
1573 http://www.bard.nih.gov/ontology/bard#BARD_0001630 promoter gene BARD> assay protocol> assay component> assay component type> biological component> biological component type> deoxyribonucleic acid> expressed component> expressed protein> promoter gene
495 http://www.bard.nih.gov/ontology/bard#BARD_0000495 reporter gene BARD> assay protocol> assay component> assay component type> biological component> biological component type> deoxyribonucleic acid> expressed component> expressed protein> reporter gene
A gene that is attached to a regulatory sequence of another gene of interest and introduced into cultured cells, animals, or plants. Certain genes function as reporters because they are easily identified and measured, or because they are selectable markers. Common reporter genes are luciferase, green fluorescent protein (GFP), beta-galactosidase, and chloramphenicol acetyltransferase (CAT).
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002656
383 http://www.bard.nih.gov/ontology/bard#BARD_0000381 gene form BARD> assay protocol> assay component> assay component type> biological component> biological component type> deoxyribonucleic acid> gene form
BioAssay Ontology v2 : http://www.bioassayontology.org/bao2.0_development#BAO_0002049
449 http://www.bard.nih.gov/ontology/bard#BARD_0000448 mutated gene BARD> assay protocol> assay component> assay component type> biological component> biological component type> deoxyribonucleic acid> gene form> mutated gene
A gene containing a mutation, defined as any alteration in a gene, and a mutation may be disease-causing or a normal variant. Mutations are introduced into cDNA that encode proteins of interest. Different types of mutations include missense, nonsense, deletions, and insertions.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000643
509 http://www.bard.nih.gov/ontology/bard#BARD_0000509 deleted gene BARD> assay protocol> assay component> assay component type> biological component> biological component type> deoxyribonucleic acid> gene form> mutated gene> deleted gene
The loss of one or more nucleotides in the genetic material (DNA or RNA), either due to a mutation or by genetic engineering. Deletion of genetic material can cause serious genetic diseases.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000230
510 http://www.bard.nih.gov/ontology/bard#BARD_0000510 inserted gene BARD> assay protocol> assay component> assay component type> biological component> biological component type> deoxyribonucleic acid> gene form> mutated gene> inserted gene
The addition of one or more nucleotides into the genetic material (DNA or RNA), which can lead to a frameshift during translation. This generates a missense or nonsense mutation, and such a protein is often non-functional.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000641
511 http://www.bard.nih.gov/ontology/bard#BARD_0000511 point-mutated gene BARD> assay protocol> assay component> assay component type> biological component> biological component type> deoxyribonucleic acid> gene form> mutated gene> point-mutated gene
The replacement of a single nucleotide with a different nucleotide in the genetic material (DNA or RNA). It results in missense, nonsense, or silent mutations.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000642
450 http://www.bard.nih.gov/ontology/bard#BARD_0000449 wild-type gene BARD> assay protocol> assay component> assay component type> biological component> biological component type> deoxyribonucleic acid> gene form> wild-type gene
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002157
384 http://www.bard.nih.gov/ontology/bard#BARD_0000382 macromolecule BARD> assay protocol> assay component> assay component type> biological component> biological component type> deoxyribonucleic acid> macromolecule
Biology term that describes a protein, nucleic acid, lipid, or carbohydrate.
Open Biomedical Ontologies : http://purl.obolibrary.org/obo/ID_0000289
871 http://www.bard.nih.gov/ontology/bard#BARD_0001395 macromolecule description BARD> assay protocol> assay component> assay component type> biological component> biological component type> deoxyribonucleic acid> macromolecule> macromolecule description
A long name for a gene or protein from a trusted international source (e.g., Entrez, UniProt).
Open Biomedical Ontologies : http://purl.obolibrary.org/obo/ID_0000294
868 http://www.bard.nih.gov/ontology/bard#BARD_0001393 macromolecule identifier BARD> assay protocol> assay component> assay component type> biological component> biological component type> deoxyribonucleic acid> macromolecule> macromolecule identifier
An external database unique identifier, such as an accession number, for a gene or protein from a trusted international source (e.g., Entrez, UniProt).
Open Biomedical Ontologies : http://purl.obolibrary.org/obo/ID_0000290
1504 http://www.bard.nih.gov/ontology/bard#BARD_0001680 GO gene-product ID BARD> assay protocol> assay component> assay component type> biological component> biological component type> deoxyribonucleic acid> macromolecule> macromolecule identifier> GO gene-product ID
1795 http://www.bard.nih.gov/ontology/bard#BARD_0001691 NCBI accession number BARD> assay protocol> assay component> assay component type> biological component> biological component type> deoxyribonucleic acid> macromolecule> macromolecule identifier> NCBI accession number
1398 http://www.bard.nih.gov/ontology/bard#BARD_0001679 UniProt accession number BARD> assay protocol> assay component> assay component type> biological component> biological component type> deoxyribonucleic acid> macromolecule> macromolecule identifier> UniProt accession number
880 http://www.bard.nih.gov/ontology/bard#BARD_0000418 gene Entrez GI BARD> assay protocol> assay component> assay component type> biological component> biological component type> deoxyribonucleic acid> macromolecule> macromolecule identifier> gene Entrez GI
2414 http://bard.broadinstitute.org/bard#2414 nucleotide Entrez ID BARD> assay protocol> assay component> assay component type> biological component> biological component type> deoxyribonucleic acid> macromolecule> macromolecule identifier> nucleotide Entrez ID
The ID of a sequence in the entrez nucleotide database.
882 http://www.bard.nih.gov/ontology/bard#BARD_0001572 protein Entrez GI BARD> assay protocol> assay component> assay component type> biological component> biological component type> deoxyribonucleic acid> macromolecule> macromolecule identifier> protein Entrez GI
872 http://www.bard.nih.gov/ontology/bard#BARD_0001204 macromolecule name BARD> assay protocol> assay component> assay component type> biological component> biological component type> deoxyribonucleic acid> macromolecule> macromolecule name
A short symbol or name for a gene or protein from a trusted international source (e.g., Entrez, UniProt).
Open Biomedical Ontologies : http://purl.obolibrary.org/obo/ID_0000292
873 http://www.bard.nih.gov/ontology/bard#BARD_0001194 macromolecule sequence BARD> assay protocol> assay component> assay component type> biological component> biological component type> deoxyribonucleic acid> macromolecule> macromolecule sequence
870 http://www.bard.nih.gov/ontology/bard#BARD_0001193 macromolecule type BARD> assay protocol> assay component> assay component type> biological component> biological component type> deoxyribonucleic acid> macromolecule> macromolecule type
Open Biomedical Ontologies : http://purl.obolibrary.org/obo/ID_0000295
1316 http://www.bard.nih.gov/ontology/bard#BARD_0001583 antibody BARD> assay protocol> assay component> assay component type> biological component> biological component type> deoxyribonucleic acid> macromolecule> macromolecule type> antibody
1774 http://www.bard.nih.gov/ontology/bard#BARD_0001675 gene BARD> assay protocol> assay component> assay component type> biological component> biological component type> deoxyribonucleic acid> macromolecule> macromolecule type> gene
884 http://www.bard.nih.gov/ontology/bard#BARD_0001573 nucleotide BARD> assay protocol> assay component> assay component type> biological component> biological component type> deoxyribonucleic acid> macromolecule> macromolecule type> nucleotide
889 http://www.bard.nih.gov/ontology/bard#BARD_0001416 peptide BARD> assay protocol> assay component> assay component type> biological component> biological component type> deoxyribonucleic acid> macromolecule> macromolecule type> peptide
1785 http://www.bard.nih.gov/ontology/bard#BARD_0001676 protein BARD> assay protocol> assay component> assay component type> biological component> biological component type> deoxyribonucleic acid> macromolecule> macromolecule type> protein
A long polymer of amino acids joined together by peptide bonds (also, polypeptide). The sequence of the amino acids is defined by the genetic code.
1784 http://www.bard.nih.gov/ontology/bard#BARD_0001575 protein-DNA complex BARD> assay protocol> assay component> assay component type> biological component> biological component type> deoxyribonucleic acid> macromolecule> macromolecule type> protein-DNA complex
1314 http://www.bard.nih.gov/ontology/bard#BARD_0001581 protein-reporter complex BARD> assay protocol> assay component> assay component type> biological component> biological component type> deoxyribonucleic acid> macromolecule> macromolecule type> protein-reporter complex
2294 http://bard.broadinstitute.org/bard#2294 TORC1 BARD> assay protocol> assay component> assay component type> biological component> biological component type> deoxyribonucleic acid> macromolecule> macromolecule type> protein> TORC1
1471 http://www.bard.nih.gov/ontology/bard#BARD_0001610 purified DNA BARD> assay protocol> assay component> assay component type> biological component> biological component type> deoxyribonucleic acid> purified DNA
An assay component type where DNA is isolated and seperated from proteins by a plasmid used to clone the desired DNA.
385 http://www.bard.nih.gov/ontology/bard#BARD_0000383 regulatory region BARD> assay protocol> assay component> assay component type> biological component> biological component type> deoxyribonucleic acid> regulatory region
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0003019
472 http://www.bard.nih.gov/ontology/bard#BARD_0000472 artificial regulatory region BARD> assay protocol> assay component> assay component type> biological component> biological component type> deoxyribonucleic acid> regulatory region> artificial regulatory region
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0003021
473 http://www.bard.nih.gov/ontology/bard#BARD_0000473 foreign regulatory region BARD> assay protocol> assay component> assay component type> biological component> biological component type> deoxyribonucleic acid> regulatory region> foreign regulatory region
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0003022
474 http://www.bard.nih.gov/ontology/bard#BARD_0000474 native regulatory region BARD> assay protocol> assay component> assay component type> biological component> biological component type> deoxyribonucleic acid> regulatory region> native regulatory region
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0003023
386 http://www.bard.nih.gov/ontology/bard#BARD_0000384 selectable marker BARD> assay protocol> assay component> assay component type> biological component> biological component type> deoxyribonucleic acid> selectable marker
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0003068
248 http://www.bard.nih.gov/ontology/bard#BARD_0000246 protein preparation BARD> assay protocol> assay component> assay component type> biological component> biological component type> protein preparation
Method by which a protein is purified from its source. The protein is a perturbagen whereby its ability to modulate a physiological process or a disease pathology is assessed.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000175
384 http://www.bard.nih.gov/ontology/bard#BARD_0000382 macromolecule BARD> assay protocol> assay component> assay component type> biological component> biological component type> protein preparation> macromolecule
Biology term that describes a protein, nucleic acid, lipid, or carbohydrate.
Open Biomedical Ontologies : http://purl.obolibrary.org/obo/ID_0000289
871 http://www.bard.nih.gov/ontology/bard#BARD_0001395 macromolecule description BARD> assay protocol> assay component> assay component type> biological component> biological component type> protein preparation> macromolecule> macromolecule description
A long name for a gene or protein from a trusted international source (e.g., Entrez, UniProt).
Open Biomedical Ontologies : http://purl.obolibrary.org/obo/ID_0000294
868 http://www.bard.nih.gov/ontology/bard#BARD_0001393 macromolecule identifier BARD> assay protocol> assay component> assay component type> biological component> biological component type> protein preparation> macromolecule> macromolecule identifier
An external database unique identifier, such as an accession number, for a gene or protein from a trusted international source (e.g., Entrez, UniProt).
Open Biomedical Ontologies : http://purl.obolibrary.org/obo/ID_0000290
1504 http://www.bard.nih.gov/ontology/bard#BARD_0001680 GO gene-product ID BARD> assay protocol> assay component> assay component type> biological component> biological component type> protein preparation> macromolecule> macromolecule identifier> GO gene-product ID
1795 http://www.bard.nih.gov/ontology/bard#BARD_0001691 NCBI accession number BARD> assay protocol> assay component> assay component type> biological component> biological component type> protein preparation> macromolecule> macromolecule identifier> NCBI accession number
1398 http://www.bard.nih.gov/ontology/bard#BARD_0001679 UniProt accession number BARD> assay protocol> assay component> assay component type> biological component> biological component type> protein preparation> macromolecule> macromolecule identifier> UniProt accession number
880 http://www.bard.nih.gov/ontology/bard#BARD_0000418 gene Entrez GI BARD> assay protocol> assay component> assay component type> biological component> biological component type> protein preparation> macromolecule> macromolecule identifier> gene Entrez GI
2414 http://bard.broadinstitute.org/bard#2414 nucleotide Entrez ID BARD> assay protocol> assay component> assay component type> biological component> biological component type> protein preparation> macromolecule> macromolecule identifier> nucleotide Entrez ID
The ID of a sequence in the entrez nucleotide database.
882 http://www.bard.nih.gov/ontology/bard#BARD_0001572 protein Entrez GI BARD> assay protocol> assay component> assay component type> biological component> biological component type> protein preparation> macromolecule> macromolecule identifier> protein Entrez GI
872 http://www.bard.nih.gov/ontology/bard#BARD_0001204 macromolecule name BARD> assay protocol> assay component> assay component type> biological component> biological component type> protein preparation> macromolecule> macromolecule name
A short symbol or name for a gene or protein from a trusted international source (e.g., Entrez, UniProt).
Open Biomedical Ontologies : http://purl.obolibrary.org/obo/ID_0000292
873 http://www.bard.nih.gov/ontology/bard#BARD_0001194 macromolecule sequence BARD> assay protocol> assay component> assay component type> biological component> biological component type> protein preparation> macromolecule> macromolecule sequence
870 http://www.bard.nih.gov/ontology/bard#BARD_0001193 macromolecule type BARD> assay protocol> assay component> assay component type> biological component> biological component type> protein preparation> macromolecule> macromolecule type
Open Biomedical Ontologies : http://purl.obolibrary.org/obo/ID_0000295
1316 http://www.bard.nih.gov/ontology/bard#BARD_0001583 antibody BARD> assay protocol> assay component> assay component type> biological component> biological component type> protein preparation> macromolecule> macromolecule type> antibody
1774 http://www.bard.nih.gov/ontology/bard#BARD_0001675 gene BARD> assay protocol> assay component> assay component type> biological component> biological component type> protein preparation> macromolecule> macromolecule type> gene
884 http://www.bard.nih.gov/ontology/bard#BARD_0001573 nucleotide BARD> assay protocol> assay component> assay component type> biological component> biological component type> protein preparation> macromolecule> macromolecule type> nucleotide
889 http://www.bard.nih.gov/ontology/bard#BARD_0001416 peptide BARD> assay protocol> assay component> assay component type> biological component> biological component type> protein preparation> macromolecule> macromolecule type> peptide
1785 http://www.bard.nih.gov/ontology/bard#BARD_0001676 protein BARD> assay protocol> assay component> assay component type> biological component> biological component type> protein preparation> macromolecule> macromolecule type> protein
A long polymer of amino acids joined together by peptide bonds (also, polypeptide). The sequence of the amino acids is defined by the genetic code.
1784 http://www.bard.nih.gov/ontology/bard#BARD_0001575 protein-DNA complex BARD> assay protocol> assay component> assay component type> biological component> biological component type> protein preparation> macromolecule> macromolecule type> protein-DNA complex
1314 http://www.bard.nih.gov/ontology/bard#BARD_0001581 protein-reporter complex BARD> assay protocol> assay component> assay component type> biological component> biological component type> protein preparation> macromolecule> macromolecule type> protein-reporter complex
2294 http://bard.broadinstitute.org/bard#2294 TORC1 BARD> assay protocol> assay component> assay component type> biological component> biological component type> protein preparation> macromolecule> macromolecule type> protein> TORC1
420 http://www.bard.nih.gov/ontology/bard#BARD_0000419 protein form BARD> assay protocol> assay component> assay component type> biological component> biological component type> protein preparation> protein form
Describes whether changes were introduced into a protein sequence and the type of changes, e.g., fusion, mutation, deletion, post-translational modifications.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000013
1659 http://www.bard.nih.gov/ontology/bard#BARD_0001635 aggregated protein BARD> assay protocol> assay component> assay component type> biological component> biological component type> protein preparation> protein form> aggregated protein
BioAssay Ontology v2 : http://www.bioassayontology.org/bao2.0_development#BAO_0002650
464 http://www.bard.nih.gov/ontology/bard#BARD_0000464 modified protein BARD> assay protocol> assay component> assay component type> biological component> biological component type> protein preparation> protein form> modified protein
Describes the type of changes that were introduced into a protein coding sequence prior to being used in the bioassay, e.g., introducing mutations, deletions, or fusion to another protein coding sequence, etc.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000354
1382 http://www.bard.nih.gov/ontology/bard#BARD_0001587 chemically labeled protein BARD> assay protocol> assay component> assay component type> biological component> biological component type> protein preparation> protein form> modified protein> chemically labeled protein
506 http://www.bard.nih.gov/ontology/bard#BARD_0000506 fused protein BARD> assay protocol> assay component> assay component type> biological component> biological component type> protein preparation> protein form> modified protein> fused protein
Fusion proteins or chimeric proteins are created through the joining of two or more cDNAs which encode different proteins. Upon expression, a single polypeptide containing each of the proteins results. Generally, the protein of interest is tagged with a reporter protein, such as luciferase or GFP, whose expression can be monitored.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000228
507 http://www.bard.nih.gov/ontology/bard#BARD_0000507 mutated protein BARD> assay protocol> assay component> assay component type> biological component> biological component type> protein preparation> protein form> modified protein> mutated protein
Mutated proteins are created by introducing mutations into the cDNAs that encode the proteins of interest and introducing those cDNA constructs into cells for expression. Different types of mutations include missense, nonsense, deletions, and insertions.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000643
525 http://www.bard.nih.gov/ontology/bard#BARD_0000525 amino acid substitution BARD> assay protocol> assay component> assay component type> biological component> biological component type> protein preparation> protein form> modified protein> mutated protein> amino acid substitution
Mutation introduced into a gene of interest, where one or more amino acids are replaced by different ones to study the role of those amino acids in the function of the protein.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000673
526 http://www.bard.nih.gov/ontology/bard#BARD_0000526 amino acid truncation BARD> assay protocol> assay component> assay component type> biological component> biological component type> protein preparation> protein form> modified protein> mutated protein> amino acid truncation
Mutation introduced into a gene of interest that either truncates the transcription of the gene or alters the gene expression so that the protein product is missing a portion of the protein towards the carboxyl-terminal. A truncated protein is generally non-functional.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000644
508 http://www.bard.nih.gov/ontology/bard#BARD_0000508 phosphorylated protein BARD> assay protocol> assay component> assay component type> biological component> biological component type> protein preparation> protein form> modified protein> phosphorylated protein
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002007
465 http://www.bard.nih.gov/ontology/bard#BARD_0000465 wild-type protein BARD> assay protocol> assay component> assay component type> biological component> biological component type> protein preparation> protein form> wild-type protein
Describes an unmodified protein.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002157
421 http://www.bard.nih.gov/ontology/bard#BARD_0000420 protein preparation method BARD> assay protocol> assay component> assay component type> biological component> biological component type> protein preparation> protein preparation method
Method by which a protein is purified from its source.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000356
466 http://www.bard.nih.gov/ontology/bard#BARD_0000466 extraction from natural source BARD> assay protocol> assay component> assay component type> biological component> biological component type> protein preparation> protein preparation method> extraction from natural source
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002076
467 http://www.bard.nih.gov/ontology/bard#BARD_0000467 recombinant expression BARD> assay protocol> assay component> assay component type> biological component> biological component type> protein preparation> protein preparation method> recombinant expression
The cDNA of the gene of interest is cloned in a plasmid or viral vector and introduced it into a bacterial, insect, or mammalian cell for expression. The cells are lysed, and the expressed protein is purified, followed by a series of centrifugation and/or chromatography and electrophoresis methods.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000360
468 http://www.bard.nih.gov/ontology/bard#BARD_0000468 synthesis by chemical process BARD> assay protocol> assay component> assay component type> biological component> biological component type> protein preparation> protein preparation method> synthesis by chemical process
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002077
422 http://www.bard.nih.gov/ontology/bard#BARD_0000421 protein purity BARD> assay protocol> assay component> assay component type> biological component> biological component type> protein preparation> protein purity
The steps to isolate a protein from either its natural or recombinantly-expressed sources. It involves lysis by either mechanical or chemical means, followed by a series of centrifugation and/or chromatography and electrophoresis methods.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002618
469 http://www.bard.nih.gov/ontology/bard#BARD_0000469 partially purified protein BARD> assay protocol> assay component> assay component type> biological component> biological component type> protein preparation> protein purity> partially purified protein
Describes an incompletely purified protein.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000570
470 http://www.bard.nih.gov/ontology/bard#BARD_0000470 purified protein BARD> assay protocol> assay component> assay component type> biological component> biological component type> protein preparation> protein purity> purified protein
Describes a fully purified protein.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0003069
471 http://www.bard.nih.gov/ontology/bard#BARD_0000471 unpurified protein sample BARD> assay protocol> assay component> assay component type> biological component> biological component type> protein preparation> protein purity> unpurified protein sample
Crude protein preparation.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000465
423 http://www.bard.nih.gov/ontology/bard#BARD_0000422 protein sequence BARD> assay protocol> assay component> assay component type> biological component> biological component type> protein preparation> protein sequence
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002818
163 http://www.bard.nih.gov/ontology/bard#BARD_0000161 species name BARD> assay protocol> assay component> assay component type> biological component> species name
66 http://www.bard.nih.gov/ontology/bard#BARD_0000064 small-molecule component BARD> assay protocol> assay component> assay component type> small-molecule component
Assay component type that describes a material of defined chemical structure, often prepared synthetically or isolated as a defined molecular entity, typically with a molecular weight of 1500 daltons or less.
Open Biomedical Ontologies : http://purl.obolibrary.org/obo/ID_0000032
207 http://www.bard.nih.gov/ontology/bard#BARD_0000205 molecular entity BARD> assay protocol> assay component> assay component type> small-molecule component> molecular entity
208 http://www.bard.nih.gov/ontology/bard#BARD_0000206 small molecule BARD> assay protocol> assay component> assay component type> small-molecule component> small molecule
891 http://www.bard.nih.gov/ontology/bard#BARD_0001418 CAS registry BARD> assay protocol> assay component> assay component type> small-molecule component> small molecule> CAS registry
878 http://www.bard.nih.gov/ontology/bard#BARD_0001205 PubChem CID BARD> assay protocol> assay component> assay component type> small-molecule component> small molecule> PubChem CID
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_000039
879 http://www.bard.nih.gov/ontology/bard#BARD_0001200 PubChem SID BARD> assay protocol> assay component> assay component type> small-molecule component> small molecule> PubChem SID
1476 http://www.bard.nih.gov/ontology/bard#BARD_0001611 QC analyst BARD> assay protocol> assay component> assay component type> small-molecule component> small molecule> QC analyst
the lab or source that Qced this substance. Should be a Compound annotation, not a result annotation
874 http://www.bard.nih.gov/ontology/bard#BARD_0001195 small-molecule name BARD> assay protocol> assay component> assay component type> small-molecule component> small molecule> small-molecule name
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000176
1775 http://www.bard.nih.gov/ontology/bard#BARD_0001681 probe identifier BARD> assay protocol> assay component> assay component type> small-molecule component> small molecule> small-molecule name> probe identifier
875 http://www.bard.nih.gov/ontology/bard#BARD_0001196 small-molecule structure BARD> assay protocol> assay component> assay component type> small-molecule component> small molecule> small-molecule structure
BioAssay Ontology v2 : http://www.bioassayontology.org/bao2.0_development#BAO_000205
876 http://www.bard.nih.gov/ontology/bard#BARD_0001197 InChI BARD> assay protocol> assay component> assay component type> small-molecule component> small molecule> small-molecule structure> InChI
BioAssay Ontology v2 : http://www.bioassayontology.org/bao2.0_development#BAO_000206
877 http://www.bard.nih.gov/ontology/bard#BARD_0001198 InChI key BARD> assay protocol> assay component> assay component type> small-molecule component> small molecule> small-molecule structure> InChI key
890 http://www.bard.nih.gov/ontology/bard#BARD_0001417 SMILES BARD> assay protocol> assay component> assay component type> small-molecule component> small molecule> small-molecule structure> SMILES
1423 http://www.bard.nih.gov/ontology/bard#BARD_0001596 solid phase bead BARD> assay protocol> assay component> assay component type> solid phase bead
An assay component type where molecules are bound on a bead and synthesized in a reactant solution.
5 http://www.bard.nih.gov/ontology/bard#BARD_0000003 assay design BARD> assay protocol> assay design
The physical parameters associated with executing and measuring the assay; defines how the assay components are used to generate data.
Open Biomedical Ontologies : http://purl.obolibrary.org/obo/ID_0000010
13 http://www.bard.nih.gov/ontology/bard#BARD_0000011 assay method BARD> assay protocol> assay design> assay method
The underlying method/technology and assay strategy used to determine the action of the perturbagen in the assay system.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002202
67 http://www.bard.nih.gov/ontology/bard#BARD_0000065 cell movement measurement method BARD> assay protocol> assay design> assay method> cell movement measurement method
Technology used to measure the movement of cells and organelles as detected by microscopy.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000499
68 http://www.bard.nih.gov/ontology/bard#BARD_0000066 conformation determination method BARD> assay protocol> assay design> assay method> conformation determination method
Assay method that uses technology to measure protein and nucleic acid conformational properties. Proteins undergo changes to their tertiary structure upon heating or binding to perturbagens, other proteins, and substrates. Nucleic acids have characteristic secondary structures, which include the B-family of structures, A-form, Z-form, guanine quadruplexes, cytosine quadruplexes, triplexes, etc. Information about conformational properties of protein and nucleic acids can be obtained by several methods, including circular dichroism spectroscopy, thermal shift, fluorescence resonance energy transfer (FRET), nuclear magnetic resonance (NMR), and X-ray crystallography.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000048
171 http://www.bard.nih.gov/ontology/bard#BARD_0000169 nucleic acid conformation assessment BARD> assay protocol> assay design> assay method> conformation determination method> nucleic acid conformation assessment
Technology that measures nucleic acid conformational properties using one of the following methods: circular dichroism spectroscopy, thermal shift, fluorescence resonance energy transfer (FRET), nuclear magnetic resonance (NMR), X-ray crystallography, or similar methods.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000469
172 http://www.bard.nih.gov/ontology/bard#BARD_0000170 protein conformation assessment BARD> assay protocol> assay design> assay method> conformation determination method> protein conformation assessment
Technology that measures protein conformational properties using one of the following methods: circular dichroism spectroscopy, thermal shift, fluorescence resonance energy transfer (FRET), nuclear magnetic resonance (NMR), or X-ray crystallography.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000470
69 http://www.bard.nih.gov/ontology/bard#BARD_0000067 cytokine quantitation method BARD> assay protocol> assay design> assay method> cytokine quantitation method
Assay method that allows direct detection of PCR-product formation by measuring the increase in fluorescent emission continuously during the PCR reaction.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002017
70 http://www.bard.nih.gov/ontology/bard#BARD_0000068 enzyme activity measurement method BARD> assay protocol> assay design> assay method> enzyme activity measurement method
Assay method that uses technology to measure an enzyme's activity, either directly or by coupling to a secondary signal.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000124
181 http://www.bard.nih.gov/ontology/bard#BARD_0000179 product formation method BARD> assay protocol> assay design> assay method> enzyme activity measurement method> product formation method
Assay format in which enzyme activity in the assay is assessed by measuring the reaction product. The product is measured by detecting the fluorophore tagged to it or by its absorbance.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000131
182 http://www.bard.nih.gov/ontology/bard#BARD_0000180 substrate depletion method BARD> assay protocol> assay design> assay method> enzyme activity measurement method> substrate depletion method
Assay format in which the enzyme activity in the assay is assessed by measuring the reduction of substrate. The substrate is measured by detecting the fluorophore tagged to it or by its absorbance.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000296
71 http://www.bard.nih.gov/ontology/bard#BARD_0000069 gene-expression detection method BARD> assay protocol> assay design> assay method> gene-expression detection method
Assay method where technology is used to quantify the expression level of the entire gene pool in a cell or a subpopulation (e.g., tissue-specific gene expression, kinases pool, etc.) using one of the three methods: microarray, bead based method or RNASeq.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0003024
183 http://www.bard.nih.gov/ontology/bard#BARD_0000181 DNA-coated bead-based method BARD> assay protocol> assay design> assay method> gene-expression detection method> DNA-coated bead-based method
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002022
184 http://www.bard.nih.gov/ontology/bard#BARD_0000182 nucleic acid amplification method BARD> assay protocol> assay design> assay method> gene-expression detection method> nucleic acid amplification method
Technology that uses purified enzymes to isolate and then replicate specific nucleic acids to levels where they can be detected. Examples are PCR, TMA, and NASBA.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002450
335 http://www.bard.nih.gov/ontology/bard#BARD_0000333 polymerase chain reaction BARD> assay protocol> assay design> assay method> gene-expression detection method> nucleic acid amplification method> polymerase chain reaction
Technique to amplify a single or few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. The polymerase chain reaction (PCR) method relies on thermal cycling, consisting of cycles of repeated heating and cooling of the reaction for DNA melting and enzymatic replication of the DNA. Primers (short DNA fragments) containing sequences complementary to the target region along with a DNA polymerase (after which the method is named) are key components to enable selective and repeated amplification. As PCR progresses, the DNA generated is itself used as a template for replication, setting in motion a chain reaction in which the DNA template is exponentially amplified.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002080
415 http://www.bard.nih.gov/ontology/bard#BARD_0000413 real-time polymerase chain reaction BARD> assay protocol> assay design> assay method> gene-expression detection method> nucleic acid amplification method> polymerase chain reaction> real-time polymerase chain reaction
Technique based on the polymerase chain reaction (PCR), and also called quantitative real time polymerase chain reaction (Q-PCR/qPCR) or kinetic polymerase chain reaction. It enables both detection and quantification (as absolute number of copies or relative amount when normalized to DNA input or additional normalizing genes) of one or more specific sequences in a DNA sample.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002084
416 http://www.bard.nih.gov/ontology/bard#BARD_0000414 reverse transcription polymerase chain reaction BARD> assay protocol> assay design> assay method> gene-expression detection method> nucleic acid amplification method> polymerase chain reaction> reverse transcription polymerase chain reaction
Reverse transcription polymerase chain reaction (RT-PCR) is a variant of the polymerase chain reaction (PCR) in which the RNA strand is first reverse-transcribed into its DNA complement (complementary DNA, or cDNA) using the enzyme reverse transcriptase, and the resulting cDNA is amplified using traditional or real-time PCR.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002089
185 http://www.bard.nih.gov/ontology/bard#BARD_0000183 nucleic acid array method BARD> assay protocol> assay design> assay method> gene-expression detection method> nucleic acid array method
Technology in which gene-specific DNA is immobilized by spotting on a solid support, such as a glass slide. The DNA is hybridized with labeled nucleic acids from a sample and the signals measured, which reflect the composition of the sample nucleic acids. This is performed to quantify gene expression under various situations,e.g., diseases, development, etc.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0003026
186 http://www.bard.nih.gov/ontology/bard#BARD_0000184 nucleic acid sequencing method BARD> assay protocol> assay design> assay method> gene-expression detection method> nucleic acid sequencing method
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002447
336 http://www.bard.nih.gov/ontology/bard#BARD_0000334 RNA-seq method BARD> assay protocol> assay design> assay method> gene-expression detection method> nucleic acid sequencing method> RNA-seq method
An approach for transcriptome profiling that uses deep-sequencing technologies. RNA-Seq, also called Whole Transcriptome Shotgun Sequencing (WTSS), refers to the use of high-throughput sequencing technologies to sequence cDNA in order to get information about a sample's RNA content. Thanks to the deep coverage and base level resolution provided by next-generation sequencing instruments, RNA-Seq provides researchers with efficient ways to measure transcriptome data experimentally, allowing them to get information such as how different alleles of a gene are expressed, detect post-transcriptional mutations, or identifying gene fusions.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0003027
187 http://www.bard.nih.gov/ontology/bard#BARD_0000185 reporter-gene method BARD> assay protocol> assay design> assay method> gene-expression detection method> reporter-gene method
Technology that uses an inducible reporter gene to study the effects of perturbagens on gene expression. Some reporter genes can be easily detected, e.g., green fluorescence protein, and others function as selectable markers, e.g., antibiotic resistance.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000125
353 http://www.bard.nih.gov/ontology/bard#BARD_0000351 beta-galactosidase induction BARD> assay protocol> assay design> assay method> gene-expression detection method> reporter-gene method> beta-galactosidase induction
Technique in which the beta galactosidase gene is attached to the regulatory sequence of a gene of interest. The effect of a perturbagen on the expression of the gene of interest can be monitored by the detection of the product of beta galactosidase enzyme.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000150
354 http://www.bard.nih.gov/ontology/bard#BARD_0000352 beta-lactamase induction BARD> assay protocol> assay design> assay method> gene-expression detection method> reporter-gene method> beta-lactamase induction
Technique in which the beta lactamase gene is attached to the regulatory sequence of a gene of interest. The effect of a perturbagen on the expression of the gene of interest can be monitored by the detection of the product of beta lactamase enzyme.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000151
355 http://www.bard.nih.gov/ontology/bard#BARD_0000353 chloramphenicol acetyltransferase induction BARD> assay protocol> assay design> assay method> gene-expression detection method> reporter-gene method> chloramphenicol acetyltransferase induction
Technique in which the chloramphenicol acetyltransferase (CAT) gene is attached to the regulatory sequence of a gene of interest. When induced to express, CAT breaks down the antibiotic chloramphenicol, resulting in a resistance against that antibiotic. The effect of a perturbagen on the expression of the gene of interest can be monitored by the gain of antibiotic resistance in the cells.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000494
356 http://www.bard.nih.gov/ontology/bard#BARD_0000354 fluorescent protein induction BARD> assay protocol> assay design> assay method> gene-expression detection method> reporter-gene method> fluorescent protein induction
Technique in which fluorescent proteins are used as reporter genes to study the effect of perturbagens on gene expression. Examples include green fluorescent protein (GFP) from the jellyfish Aequorea victoria and the various modifications of it created by amino acid substitutions to either optimize the codon usage in mammalian cells or to change the spectral properties of the protein.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000153
357 http://www.bard.nih.gov/ontology/bard#BARD_0000355 luciferase induction BARD> assay protocol> assay design> assay method> gene-expression detection method> reporter-gene method> luciferase induction
Technique in which the luciferase gene is attached to the regulatory sequence of a gene of interest. The effect of a perturbagen on the expression of the gene of interest can be measured by the detection of light emitted as a product the luciferase reaction.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000152
188 http://www.bard.nih.gov/ontology/bard#BARD_0000186 serial analysis of gene expression BARD> assay protocol> assay design> assay method> gene-expression detection method> serial analysis of gene expression
Serial analysis of gene expression (SAGE) is a technique used to produce a snapshot of the messenger RNA population in a sample of interest in the form of small tags that correspond to fragments of those transcripts.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002430
72 http://www.bard.nih.gov/ontology/bard#BARD_0000070 membrane potential measurement method BARD> assay protocol> assay design> assay method> membrane potential measurement method
Assay method that uses technology to measure the difference in voltage between the interior and exterior of the cell. Electrically active cells, such as neurons, muscle and endocrine cells undergo changes in membrane potential due to depolarization and hyperpolarization as a result of opening and closing of ion channels. Action potentials are used to transmit signals across neurons, and in muscle and endocrine cells, they lead to intracellular events, such as muscle contraction and hormone release, respectively. In addition, mitochondria possess transmembrane potentials as a result of their respiratory function. Membrane potential can be measured either by using electrophysiological techniques or by staining with a dye whose binding is dependent on the membrane potential.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000377
189 http://www.bard.nih.gov/ontology/bard#BARD_0000187 dye-binding method BARD> assay protocol> assay design> assay method> membrane potential measurement method> dye-binding method
Technology that measures the delta-psi (mitochondrial membrane potential) by staining with specific dyes whose binding is dependent on the potential.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000425
190 http://www.bard.nih.gov/ontology/bard#BARD_0000188 electrophysiological method BARD> assay protocol> assay design> assay method> membrane potential measurement method> electrophysiological method
Technology that measures changes in membrane potential via opening and closing of ion channels in electrically active cells (such as neurons, muscle cells, and endocrine cells) by the insertion or attachment of microelectrodes. Patch clamp, voltage clamp, and current clamp are three ways of studying the current-voltage relationships of the ion channels.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000424
73 http://www.bard.nih.gov/ontology/bard#BARD_0000071 molecular interaction assessment method BARD> assay protocol> assay design> assay method> molecular interaction assessment method
A type of assay method where covalent interactions hold the atoms of biopolymers and small molecules together.
BioAssay Ontology v2 : http://www.bioassayontology.org/bao2.0_development#BAO_0002205
191 http://www.bard.nih.gov/ontology/bard#BARD_0000189 binding assessment method BARD> assay protocol> assay design> assay method> molecular interaction assessment method> binding assessment method
Technology is used to quantitate the interactions between two molecules, e.g., perturbagen-protein, protein-protein, protein-DNA, etc. Methods used include radioligand binding, resonance energy transfer, fluorescence polarization, protein fragment complementation assay, and several label-free methods, as well as surface plasmon resonance, impedance, nuclear magnetic resonance, X-ray diffraction, isothermal titration calorimetry, and mass spectrometry.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000123
234 http://www.bard.nih.gov/ontology/bard#BARD_0000232 ex situ immunoassay BARD> assay protocol> assay design> assay method> molecular interaction assessment method> binding assessment method> ex situ immunoassay
Measures the concentration of a substance in a solution based on the specific binding of an antibody to that substance, which produces a measurable signal. The signal measured depends on the label that is associated with this interaction, such as radioactive (in radioimmunoassays), fluorescent, phosphorescent, and chemiluminescent dyes, etc.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000129
390 http://www.bard.nih.gov/ontology/bard#BARD_0000388 dot immunobinding assay BARD> assay protocol> assay design> assay method> molecular interaction assessment method> binding assessment method> ex situ immunoassay> dot immunobinding assay
In the dot immunobinding assay (Dot Iba) immunodiagnostic method, antibody or antigen is dotted directly onto nitrocellulose membrane (NCM) discs. The diagnostic material to be checked can be incubated on this disc. Presence of antigen antibody complex in NCM discs is directly demonstrated with enzyme-conjugated antiglobulins and substrate. Development of a purple-pink insoluble substrate product in the NCM is a positive result in the assay. This assay allows the processing of multiple specimens at a time.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002415
391 http://www.bard.nih.gov/ontology/bard#BARD_0000389 enzyme-linked immunosorbent assay BARD> assay protocol> assay design> assay method> molecular interaction assessment method> binding assessment method> ex situ immunoassay> enzyme-linked immunosorbent assay
Enzyme-linked immunosorbent assay (ELISA) is used to detect the presence of a particular protein, either an antigen or an antibody, in a sample, such as a body fluid.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000134
441 http://www.bard.nih.gov/ontology/bard#BARD_0000440 antigen down assay BARD> assay protocol> assay design> assay method> molecular interaction assessment method> binding assessment method> ex situ immunoassay> enzyme-linked immunosorbent assay> antigen down assay
Technology that binds an antigen to a solid surface instead of to an antibody. Also called an immunometric assay, this assay is used to bind antibodies found in a sample. When a sample (such as human serum) is added, the antibodies (IgE for example) from the sample bind to the antigen coated on the plate.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000137
442 http://www.bard.nih.gov/ontology/bard#BARD_0000441 chemiluminescence-linked immunosorbent assay BARD> assay protocol> assay design> assay method> molecular interaction assessment method> binding assessment method> ex situ immunoassay> enzyme-linked immunosorbent assay> chemiluminescence-linked immunosorbent assay
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002417
443 http://www.bard.nih.gov/ontology/bard#BARD_0000442 competitive immunoassay BARD> assay protocol> assay design> assay method> molecular interaction assessment method> binding assessment method> ex situ immunoassay> enzyme-linked immunosorbent assay> competitive immunoassay
Technology based on the competition of labeled and unlabeled ligand for a limited number of antibody binding sites. The free antigen and antibody are incubated to form antigen-antibody complex and then the complex is bound to the antigen-coated surface of the assay plate. The unbound antibody-antigen complex is washed off before adding an enzyme-linked secondary antibody against the primary antibody. The substrate is then added and the antigen concentration can be determined by the signal strength elicited by the enzyme-substrate reaction. In this assay, the enzyme-linked secondary antibody competes with the sample antigen that is associated with the primary antibody.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000132
444 http://www.bard.nih.gov/ontology/bard#BARD_0000443 enzyme-linked immunosorbent spot assay BARD> assay protocol> assay design> assay method> molecular interaction assessment method> binding assessment method> ex situ immunoassay> enzyme-linked immunosorbent assay> enzyme-linked immunosorbent spot assay
Technology based on the enzyme-linked immunosorbent assay (ELISA) immunosassay. Enzyme-linked immunosorbent spot (ELISPOT) assays were originally developed to enumerate B cells secreting antigen-specific antibodies, and have subsequently been adapted for other purposes, especially the identification and enumeration of cytokine-producing cells at the single cell level. Under appropriate conditions, the ELISPOT assay allows visualization of the secretory product of individual activated or responding cells. Each spot that develops in the assay represents a single reactive cell. Thus, the ELISPOT assay provides both qualitative (type of immune protein) and quantitative (number of responding cells) information.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002419
445 http://www.bard.nih.gov/ontology/bard#BARD_0000444 fluorescence-linked immunosorbent assay BARD> assay protocol> assay design> assay method> molecular interaction assessment method> binding assessment method> ex situ immunoassay> enzyme-linked immunosorbent assay> fluorescence-linked immunosorbent assay
Technology using a fluorescent probe on an antibody of interest, resulting in direct detection of fluorescence intensity. Compared with the sandwich enzyme-linked immunosorbent assay (ELISA), the fluorescence-linked immunosorbent assay (FLISA) has similar sensitivity and specificity, but it is much more rapid, economic, and convenient.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002420
446 http://www.bard.nih.gov/ontology/bard#BARD_0000445 sandwich enzyme-linked immunosorbent assay BARD> assay protocol> assay design> assay method> molecular interaction assessment method> binding assessment method> ex situ immunoassay> enzyme-linked immunosorbent assay> sandwich enzyme-linked immunosorbent assay
Technology that measures the amount of antigen between two layers of antibodies. The antigens to be measured must contain at least two antigenic sites capable of binding to antibodies, since at least two antibodies act in the sandwich, so sandwich assays are restricted to the quantitation of multivalent antigens such as proteins or polysaccharides. Sandwich enzyme-linked immunosorbent assays (sandwich ELISAs) for quantitation of antigens are especially valuable when the concentration of antigens is low and/or the antigens are contained in high concentrations of contaminating protein.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002421
392 http://www.bard.nih.gov/ontology/bard#BARD_0000390 enzyme-multiplied immunoassay technique BARD> assay protocol> assay design> assay method> molecular interaction assessment method> binding assessment method> ex situ immunoassay> enzyme-multiplied immunoassay technique
Enzyme multiplied immunoassay technique (EMIT) is a common method for screening urine and blood for drugs. A mix and read protocol has been developed that is simple and rapid. The most widely used applications for EMIT are for therapeutic drug monitoring (serum) and as a primary screen for abused drugs and their metabolites (urine).
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002401
393 http://www.bard.nih.gov/ontology/bard#BARD_0000391 immunoblot BARD> assay protocol> assay design> assay method> molecular interaction assessment method> binding assessment method> ex situ immunoassay> immunoblot
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002422
452 http://www.bard.nih.gov/ontology/bard#BARD_0000451 Western blot BARD> assay protocol> assay design> assay method> molecular interaction assessment method> binding assessment method> ex situ immunoassay> immunoblot> Western blot
An analytical technique used to detect specific proteins in a given sample of tissue homogenate or extract. Also known as protein immunoblot, it uses gel electrophoresis to separate native or denatured proteins by the length of the polypeptide (denaturing conditions) or by the 3-D structure of the protein (native/ non-denaturing conditions). The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are probed (detected) using antibodies specific to the target protein.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002424
451 http://www.bard.nih.gov/ontology/bard#BARD_0000450 in-cell Western assay BARD> assay protocol> assay design> assay method> molecular interaction assessment method> binding assessment method> ex situ immunoassay> immunoblot> in-cell Western assay
An immunocytochemical assay that uses NIR fluorescence to detect and quantify proteins in fixed cells. Detecting proteins in their cellular context increases quantification precision. Proteins in fixed, cultured cells are detected directly in microplates, which yields higher throughput compared to Western blotting and eliminates typical Western blotting steps such as cell lysate preparation, electrophoresis, and membrane transfer.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002423
394 http://www.bard.nih.gov/ontology/bard#BARD_0000392 radioimmunoassay BARD> assay protocol> assay design> assay method> molecular interaction assessment method> binding assessment method> ex situ immunoassay> radioimmunoassay
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002428
395 http://www.bard.nih.gov/ontology/bard#BARD_0000393 two-site enzyme immunoassay BARD> assay protocol> assay design> assay method> molecular interaction assessment method> binding assessment method> ex situ immunoassay> two-site enzyme immunoassay
Measures the amount of antigen in a sample. The antigen under assay which must have two or more epitopes. It is insolubilized by reaction with an unlabelled antibody conjugated to a solid phase, then reacted with an enzyme-labelled antibody directed to a different epitope of the analyte. The quantity of labelled antibody which becomes immobilized due to the complexing reaction is directly proportional to the amount of analyte present in the sample.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002411
235 http://www.bard.nih.gov/ontology/bard#BARD_0000233 protein fragment complementation assay BARD> assay protocol> assay design> assay method> molecular interaction assessment method> binding assessment method> protein fragment complementation assay
Used to study the interaction of two proteins. An enzyme or fluorescent protein is rationally dissected into two fragments and fused to two test proteins, whose interaction is being studied. When the two test proteins bind, the enzyme or fluorescent protein is reconstituted to a functional state, and that functionality can be measured.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000406
236 http://www.bard.nih.gov/ontology/bard#BARD_0000234 radioligand binding assay BARD> assay protocol> assay design> assay method> molecular interaction assessment method> binding assessment method> radioligand binding assay
Used for analysis of receptor-ligand interactions in screening applications, and measures the level of binding of a radiolabeled ligand to some receptor, which can be on cell membranes or in soluble/purified form.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000402
74 http://www.bard.nih.gov/ontology/bard#BARD_0000072 morphology assessment method BARD> assay protocol> assay design> assay method> morphology assessment method
Assay method that uses technology, detected by microscopy, to measure how the shape and form of molecules affect their chemical properties, dynamic reconfiguration and interactions.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000448
192 http://www.bard.nih.gov/ontology/bard#BARD_0000190 in situ immunoassay BARD> assay protocol> assay design> assay method> morphology assessment method> in situ immunoassay
Examines an antigen that is undisturbed or in place/position; protein detection and localization is achieved by visualization under a microscope.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000128
284 http://www.bard.nih.gov/ontology/bard#BARD_0000282 immunocytochemistry BARD> assay protocol> assay design> assay method> morphology assessment method> in situ immunoassay> immunocytochemistry
Used to detect proteins and peptides in a cell by binding to specific antibodies. Subsequently, a secondary antibody that is conjugated to a fluorophore or an enzyme needed for the detection of a signal is hybridized to the primary antibody. Signal is generally detected by immunofluorescence or less commonly, by a colored reaction. This allows the detection and subcellular localization of the protein of interest by visualization under a microscope.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000416
285 http://www.bard.nih.gov/ontology/bard#BARD_0000283 immunohistochemistry BARD> assay protocol> assay design> assay method> morphology assessment method> in situ immunoassay> immunohistochemistry
Used to detect proteins and peptides in a tissue section by binding to specific antibodies. Subsequently, a secondary antibody that is conjugated to a fluorophore or an enzyme needed for the detection of a signal is hybridized to the primary antibody. Signal is generally detected by immunofluorescence or less commonly, by a colored reaction. This allows the detection and subcellular localization of the protein of interest by visualization under a microscope.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000415
75 http://www.bard.nih.gov/ontology/bard#BARD_0000073 phosphoprotein detection method BARD> assay protocol> assay design> assay method> phosphoprotein detection method
A type of assay method that deals with physically bonded substances containing phosphoric acid.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002013
76 http://www.bard.nih.gov/ontology/bard#BARD_0000074 redistribution determination method BARD> assay protocol> assay design> assay method> redistribution determination method
Assay method that uses technology to measure the distribution of organelles and molecules within the cell (proteins, second messengers, metal ions, dyes, etc), as generally visualized by microscopy.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000126
198 http://www.bard.nih.gov/ontology/bard#BARD_0000196 dye redistribution BARD> assay protocol> assay design> assay method> redistribution determination method> dye redistribution
Used to study flux of molecules across membranes, e.g., calcein AM, ethidium homodimer-1, etc.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000653
199 http://www.bard.nih.gov/ontology/bard#BARD_0000197 ion redistribution BARD> assay protocol> assay design> assay method> redistribution determination method> ion redistribution
Used to study ion channel activity by chemically initiating the opening of channels in the presence of a fixed amount of radiolabeled cation or anion, and a potential ion channel modulator compound.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000819
200 http://www.bard.nih.gov/ontology/bard#BARD_0000198 metal ion redistribution BARD> assay protocol> assay design> assay method> redistribution determination method> metal ion redistribution
Used to study the way metal ions such as copper, iron, magnesium and zinc are transported between different cellular compartments to bring about specific functions. This is regulated by their influx and efflux across membranes, which could occur either by passive diffusion or active transport.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000162
201 http://www.bard.nih.gov/ontology/bard#BARD_0000199 protein redistribution BARD> assay protocol> assay design> assay method> redistribution determination method> protein redistribution
Used to study how cellular signaling brings about the movement of proteins in cells. Upon receiving a cue, proteins undergo posttranslational modifications, e.g., phosphorylation, dephosphorylation, etc., which results in their activation and migration to different compartments, often to the nucleus, where they regulate gene expression.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000378
202 http://www.bard.nih.gov/ontology/bard#BARD_0000200 second messenger redistribution BARD> assay protocol> assay design> assay method> redistribution determination method> second messenger redistribution
Used to study how second messengers relay and amplify signals from the receptors to the cell interior as part of a signaling event. Examples include cAMP, cGMP, calcium, nitric oxide, diacylglycerol, etc., which play important roles in different physiological processes.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000379
358 http://www.bard.nih.gov/ontology/bard#BARD_0000356 calcium redistribution BARD> assay protocol> assay design> assay method> redistribution determination method> second messenger redistribution> calcium redistribution
Assay method where calcium functions as the second messenger in cell signaling.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000649
359 http://www.bard.nih.gov/ontology/bard#BARD_0000357 cyclic-AMP redistribution BARD> assay protocol> assay design> assay method> redistribution determination method> second messenger redistribution> cyclic-AMP redistribution
Assay method where cyclic AMP (cAMP) functions as second messenger in cell signaling.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000650
360 http://www.bard.nih.gov/ontology/bard#BARD_0000358 cyclic-GMP redistribution BARD> assay protocol> assay design> assay method> redistribution determination method> second messenger redistribution> cyclic-GMP redistribution
Assay method where cyclic GMP (cGMP) functions as second messenger in cell signaling.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000651
77 http://www.bard.nih.gov/ontology/bard#BARD_0000075 viability measurement method BARD> assay protocol> assay design> assay method> viability measurement method
A type of assay method that employs technology to measure cell life or death by quantitating ATP content, caspase activity, membrane potential, and other measures. Measures the ability of a cell to maintain itself or recover its potentialities.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000127
215 http://www.bard.nih.gov/ontology/bard#BARD_0000213 ATP quantitation BARD> assay protocol> assay design> assay method> viability measurement method> ATP quantitation
ATP levels are an indicator of viable cell number. ATP levels higher than controls (untreated cells) indicate proliferation and ATP levels lower than controls indicate cytotoxicity. ATP can be quantified using different commercial kits.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000164
220 http://www.bard.nih.gov/ontology/bard#BARD_0000218 DNA content estimation BARD> assay protocol> assay design> assay method> viability measurement method> DNA content estimation
Cell nuclei can be by stained with Hoechst, DAPI, etc., and the depth of staining indicates the DNA content, which can be used as a measure of cell viability and proliferation.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000169
216 http://www.bard.nih.gov/ontology/bard#BARD_0000214 caspase activity determination BARD> assay protocol> assay design> assay method> viability measurement method> caspase activity determination
Caspase levels are an indicator of the degree of apoptosis happening in cells in a sample, and caspase levels higher than controls indicate cytotoxicity. Caspase levels can be quantified using different commercial kits.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000165
217 http://www.bard.nih.gov/ontology/bard#BARD_0000215 cell number determination BARD> assay protocol> assay design> assay method> viability measurement method> cell number determination
Cells are counted to determine their viability. Cultured animal cells are counted either manually using hemocytometer or by an automated cell counter, e.g., Coulter counter or Vi-CELL. The automated counter can distinguish live and dead cells by the trypan blue cell exclusion method. Yeast and bacterial cell growth is determined by measuring absorbance in a spectrophotometer or by viable plate count.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000572
2528 http://bard.broadinstitute.org/bard#2528 cellular protein quantitation method BARD> assay protocol> assay design> assay method> viability measurement method> cellular protein quantitation method
Method of measuring the amount of protein in cells, typically using spectrophotometric methods such as absorbance or fluorescence, and comparing to a control, standard, or established concentration curve to quantify the proliferation or level of protein expression of cells.
218 http://www.bard.nih.gov/ontology/bard#BARD_0000216 chromatin condensation visualization BARD> assay protocol> assay design> assay method> viability measurement method> chromatin condensation visualization
Visualizes the chromatin condensation morphological changes (also known as pyknosis) that occur during apoptosis by microscopic examination of dye staining.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000159
219 http://www.bard.nih.gov/ontology/bard#BARD_0000217 dehydrogenase activity determination BARD> assay protocol> assay design> assay method> viability measurement method> dehydrogenase activity determination
An expansion in the number of viable cells results in an increase in the overall activity of mitochondrial dehydrogenases in the sample. The internal environment of the proliferating cell is more reduced than that of non-proliferating cells; specifically, the ratios of NADPH/NADP+, FADH/FAD+, FMNH/FMN+, and NADH/NAD+ increase during proliferation. Compounds such as tetrazolium salts and Alamar Blue, which can be reduced by these metabolic intermediates, can be used to monitor cell proliferation. Their reduction is accompanied by a measurable shift in wavelength with a corresponding change in color.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000166
221 http://www.bard.nih.gov/ontology/bard#BARD_0000219 esterase activity determination BARD> assay protocol> assay design> assay method> viability measurement method> esterase activity determination
Cell viability can be assessed by the presence of cytoplasmic esterases that cleave a lipid-soluble nonfluorescent probe to yield a fluorescent product. The product is charged and is thus retained within the cell if the membrane is intact. Typical probes include fluorescein diacetate (FDA), carboxyfluorescein, and calcein.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000158
222 http://www.bard.nih.gov/ontology/bard#BARD_0000220 membrane permeability assessment BARD> assay protocol> assay design> assay method> viability measurement method> membrane permeability assessment
Live cells with intact membranes have the ability to exclude dyes that easily penetrate dead or damaged cells. Examples of the dyes include propidium iodide, 7-amino actinomycin D, and trypan blue. Cells stained with the former two can be quantified by flow cytometry, while the latter is quantified by manual counting using hemocytometer.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000167
223 http://www.bard.nih.gov/ontology/bard#BARD_0000221 mitochondrial membrane potential assessment BARD> assay protocol> assay design> assay method> viability measurement method> mitochondrial membrane potential assessment
Activity of the mitochondrial respiratory chain results in the generation of a proton gradient that consists of two components: a membrane potential and a pH gradient. Disruption of delta-psi (mitochondrial membrane potential) has been shown to be one of the first intracellular changes following the onset of apoptosis. Delta-psi is generally quantified by staining with specific dyes whose binding is dependent on the potential and, less commonly, by electrophysiological techniques.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000423
2405 http://bard.broadinstitute.org/bard#2405 nucleotide incorporation method BARD> assay protocol> assay design> assay method> viability measurement method> nucleotide incorporation method
224 http://www.bard.nih.gov/ontology/bard#BARD_0000222 phospholipid redistribution estimation BARD> assay protocol> assay design> assay method> viability measurement method> phospholipid redistribution estimation
In a viable cell, phosphatidyl serine (PS), a phospholipid, is located on the inner leaflet of the cell membrane facing the cytosol. When the cell is undergoing apoptosis, the cell membrane loses its phospholipid asymmetry and exposes PS on the outer layer of the membrane. Apoptotic cells can be detected by staining with fluorescein isothiocyanate (FITC)-conjugated Annexin V, which specifically binds PS, followed by flow cytometry.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000073
225 http://www.bard.nih.gov/ontology/bard#BARD_0000223 protease activity determination BARD> assay protocol> assay design> assay method> viability measurement method> protease activity determination
Live cells have a conserved and constitutive protease activity that serves as a biomarker of cell viability. The live-cell protease activity is measured using a fluorogenic, cell-permeant, peptide substrate (Gly-Phe-AFC). The substrate enters intact cells, where it is cleaved by the live-cell protease activity to generate a fluorescent signal proportional to the number of living cells. The live-cell protease becomes inactive upon loss of membrane integrity and leakage into the surrounding culture medium.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000170
14 http://www.bard.nih.gov/ontology/bard#BARD_0000012 assay parameter BARD> assay protocol> assay design> assay parameter
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000026
78 http://www.bard.nih.gov/ontology/bard#BARD_0000076 assay biosafety level BARD> assay protocol> assay design> assay parameter> assay biosafety level
An assay parameter that is the level of the biocontainment precautions that is required to isolate hazardous biological agents in an enclosed facility; range from the lowest biosafety level of 1 to the highest at level 4.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000436
137 http://www.bard.nih.gov/ontology/bard#BARD_0000135 BSL1 BARD> assay protocol> assay design> assay parameter> assay biosafety level> BSL1
Biosafety Level 1: Suitable for work involving well-characterized agents not known to consistently cause disease in immunocompetent adult humans, and present minimal potential hazard to laboratory personnel and the environment (definition from CDC).
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000437
138 http://www.bard.nih.gov/ontology/bard#BARD_0000136 BSL2 BARD> assay protocol> assay design> assay parameter> assay biosafety level> BSL2
Biosafety Level 2: Suitable for work involving agents that pose moderate hazards to personnel and the environment. It differs from BSL-1 in that 1) laboratory personnel have specific training in handling pathogenic agents and are supervised by scientists competent in handling infectious agents and associated procedures; 2) access to the laboratory is restricted when work is being conducted; and 3) all procedures in which infectious aerosols or splashes may be created are conducted in biosafety cabinets (BSCs) or other physical containment equipment (definition from CDC).
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000438
139 http://www.bard.nih.gov/ontology/bard#BARD_0000137 BSL2+ BARD> assay protocol> assay design> assay parameter> assay biosafety level> BSL2+
140 http://www.bard.nih.gov/ontology/bard#BARD_0000138 BSL3 BARD> assay protocol> assay design> assay parameter> assay biosafety level> BSL3
Biosafety Level 3: Applicable to clinical, diagnostic, teaching, research, or production facilities where work is performed with indigenous or exotic agents that may cause serious or potentially lethal disease through inhalation route exposure. Laboratory personnel must receive specific training in handling pathogenic and potentially lethal agents, and must be supervised by scientists competent in handling infectious agents and associated procedures. All procedures involving the manipulation of infectious materials must be conducted within BSCs, other physical containment devices, or by personnel wearing appropriate personal protective equipment (definition from CDC).
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000635
141 http://www.bard.nih.gov/ontology/bard#BARD_0000139 BSL4 BARD> assay protocol> assay design> assay parameter> assay biosafety level> BSL4
BioAssay Ontology v2 : http://www.bioassayontology.org/bao2.0_development#BAO_0002065
79 http://www.bard.nih.gov/ontology/bard#BARD_0000077 assay condition BARD> assay protocol> assay design> assay parameter> assay condition
A type of assay parameter that consists of a set of optimization guidelines used to minimize the time and cost of assay implementation while providing reliable assay performance.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000018
2046 http://bard.broadinstitute.org/bard#2046 assay hold voltage BARD> assay protocol> assay design> assay parameter> assay condition> assay hold voltage
voltage applied to the system under study and maintained using a feedback loop
142 http://www.bard.nih.gov/ontology/bard#BARD_0000140 assay humidity BARD> assay protocol> assay design> assay parameter> assay condition> assay humidity
145 http://www.bard.nih.gov/ontology/bard#BARD_0000143 assay pH BARD> assay protocol> assay design> assay parameter> assay condition> assay pH
The pH at which the assay was performed.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002827
143 http://www.bard.nih.gov/ontology/bard#BARD_0000141 assay percent carbon dioxide BARD> assay protocol> assay design> assay parameter> assay condition> assay percent carbon dioxide
%
144 http://www.bard.nih.gov/ontology/bard#BARD_0000142 assay percent oxygen BARD> assay protocol> assay design> assay parameter> assay condition> assay percent oxygen
%
146 http://www.bard.nih.gov/ontology/bard#BARD_0000144 assay pressure BARD> assay protocol> assay design> assay parameter> assay condition> assay pressure
The force per unit area applied in a direction perpendicular to the surface of an object in the assay.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002828
147 http://www.bard.nih.gov/ontology/bard#BARD_0000145 assay temperature BARD> assay protocol> assay design> assay parameter> assay condition> assay temperature
The temperature at which the assay was conducted.
deg C BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002832
148 http://www.bard.nih.gov/ontology/bard#BARD_0000146 incubation temperature BARD> assay protocol> assay design> assay parameter> assay condition> incubation temperature
deg C
149 http://www.bard.nih.gov/ontology/bard#BARD_0000147 incubation time BARD> assay protocol> assay design> assay parameter> assay condition> incubation time
Interval of time between the addition of pertubagen, substrate, or cell modification, and the measurement of change using the detection method of the assay.
min BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002833
286 http://www.bard.nih.gov/ontology/bard#BARD_0000284 cell modification time BARD> assay protocol> assay design> assay parameter> assay condition> incubation time> cell modification time
The interval of time between the cell modification, as by transient transfection of plasmid DNA/siRNA, viral transduction, etc., and the measurement of change, as observed by a detection method in the bioassay.
min BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002834
287 http://www.bard.nih.gov/ontology/bard#BARD_0000285 coupled substrate incubation time BARD> assay protocol> assay design> assay parameter> assay condition> incubation time> coupled substrate incubation time
The interval of time between the addition of a coupled enzyme substrate and the reaction-stopping procedure in a coupled enzyme assay.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002835
288 http://www.bard.nih.gov/ontology/bard#BARD_0000286 enzyme reaction time BARD> assay protocol> assay design> assay parameter> assay condition> incubation time> enzyme reaction time
The interval of time between the enzyme activity measurements in a kinetic assay.
min BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002836
289 http://www.bard.nih.gov/ontology/bard#BARD_0000287 perturbagen incubation time BARD> assay protocol> assay design> assay parameter> assay condition> incubation time> perturbagen incubation time
The interval of time between the addition of a perturbagen and the measurement of change, as observed by a detection/readout.
min BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002837
290 http://www.bard.nih.gov/ontology/bard#BARD_0000288 substrate incubation time BARD> assay protocol> assay design> assay parameter> assay condition> incubation time> substrate incubation time
The interval of time between the addition of an enzyme substrate and the measurement of change, as observed by a detection/readout.
min BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002838
80 http://www.bard.nih.gov/ontology/bard#BARD_0000078 assay footprint BARD> assay protocol> assay design> assay parameter> assay footprint
A type of assay parameter that describes the physical format in which an assay is performed; generally a microplate format, but can also be an array format. Represents the specific result factor involved in computing the experiment.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000512
1427 http://www.bard.nih.gov/ontology/bard#BARD_0001600 8-well square agar plates BARD> assay protocol> assay design> assay parameter> assay footprint> 8-well square agar plates
from UNM min Assay annnotaion xls
2410 http://www.bard.nih.gov/ontology/bard#BARD_0001835 Gel BARD> assay protocol> assay design> assay parameter> assay footprint> Gel
2011 http://bard.broadinstitute.org/bard#2011 Iuvo Single Microchannel Plate BARD> assay protocol> assay design> assay parameter> assay footprint> Iuvo Single Microchannel Plate
1391 http://www.bard.nih.gov/ontology/bard#BARD_0001588 Microscope cover slip 22 mm sq BARD> assay protocol> assay design> assay parameter> assay footprint> Microscope cover slip 22 mm sq
1670 http://www.bard.nih.gov/ontology/bard#BARD_0001638 NMR tube BARD> assay protocol> assay design> assay parameter> assay footprint> NMR tube
156 http://www.bard.nih.gov/ontology/bard#BARD_0000154 array BARD> assay protocol> assay design> assay parameter> assay footprint> array
The immobilization of thousands of small molecules, genes, or proteins on a glass slide for binding studies.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000514
226 http://www.bard.nih.gov/ontology/bard#BARD_0000224 nucleic acid array BARD> assay protocol> assay design> assay parameter> assay footprint> array> nucleic acid array
Nucleic acids (either DNA or RNA) are immobilized by spotting on a solid support, such as a glass slide, and are hybridized with labeled nucleic acids from a sample. The measured signals reflect the composition of the sample's nucleic acids, and quantifies gene expression under various conditions, e.g., diseases, development, etc.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000504
408 http://www.bard.nih.gov/ontology/bard#BARD_0000406 gene array BARD> assay protocol> assay design> assay parameter> assay footprint> array> nucleic acid array> gene array
Provides a collection of gene-specific nucleic acids that have been placed at defined locations on a solid support either by spotting or direct synthesis. Labeled nucleic acids from a sample are hybridized with target genes on the array and the signals measured, which reflects the composition of the sample nucleic acids.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000506
409 http://www.bard.nih.gov/ontology/bard#BARD_0000407 oligonucleotide array BARD> assay protocol> assay design> assay parameter> assay footprint> array> nucleic acid array> oligonucleotide array
Provides a collection of oligonucleotides that are immobilized by spotting on a solid support, such as a glass slide. They are hybridized with labeled nucleic acids from a sample and the signals measured, which reflects the composition of the sample nucleic acids. This is performed to either quantify gene expression or genomic profiling to understand genetic variation in humans.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000133
227 http://www.bard.nih.gov/ontology/bard#BARD_0000225 protein array BARD> assay protocol> assay design> assay parameter> assay footprint> array> protein array
Purified proteins, cell or tissue lysates, antibodies, or peptides are immobilized by spotting on a solid support, such as a glass slide, in order to quantify the proteome or study protein-protein, protein-DNA, protein-small molecule interactions, and enzyme activity.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000505
417 http://www.bard.nih.gov/ontology/bard#BARD_0000415 analytical array BARD> assay protocol> assay design> assay parameter> assay footprint> array> protein array> analytical array
Method in which antibodies or peptides are spotted on a support and hybridized to analytes in complex mixtures of proteins.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000492
418 http://www.bard.nih.gov/ontology/bard#BARD_0000416 lysate array BARD> assay protocol> assay design> assay parameter> assay footprint> array> protein array> lysate array
Contains complex samples, such as cell or tissue lysates, spotted on an array surface and interrogated with antibodies.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000474
419 http://www.bard.nih.gov/ontology/bard#BARD_0000417 purified protein array BARD> assay protocol> assay design> assay parameter> assay footprint> array> protein array> purified protein array
Method in which many different purified proteins are spotted on a support, and used to assay biochemical functions such as protein-protein, protein-DNA, protein-small molecule interactions, and enzyme activity.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000501
228 http://www.bard.nih.gov/ontology/bard#BARD_0000226 small-molecule array BARD> assay protocol> assay design> assay parameter> assay footprint> array> small-molecule array
Small molecules that are either natural or synthetic are immobilized by robotic printing on a solid support, such as a functionalized glass slide. Protein targets, either purified or from a lysate, are hybridized to the immobilized small molecules in an attempt to identify the natural ligands for proteins and molecules that might regulate their function.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000473
157 http://www.bard.nih.gov/ontology/bard#BARD_0000155 cuvette BARD> assay protocol> assay design> assay parameter> assay footprint> cuvette
Used to measure absorbance or fluorescence in a spectrophotometer or fluorimeter, respectively; there are plastic, glass, or quartz cuvettes.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000578
158 http://www.bard.nih.gov/ontology/bard#BARD_0000156 microplate BARD> assay protocol> assay design> assay parameter> assay footprint> microplate
Describes an assay footprint that is a plate, also microtiter plate or microwell plate, that has multiple sample wells arranged in a 2:3 rectangular matrix and may have anywhere from 12 wells to 1536 or more wells.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000508
313 http://www.bard.nih.gov/ontology/bard#BARD_0000311 12-well plate BARD> assay protocol> assay design> assay parameter> assay footprint> microplate> 12-well plate
Microplate containing 12 sample wells. Suitable for a low-throughput assay.
314 http://www.bard.nih.gov/ontology/bard#BARD_0000312 1536-well plate BARD> assay protocol> assay design> assay parameter> assay footprint> microplate> 1536-well plate
Microplate containing 1536 sample wells. It is highly miniaturized to accommodate more wells on one plate, which reduces the plate handling time and allows working with very small volumes (a few microliters), reducing the cost of an assay.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000516
315 http://www.bard.nih.gov/ontology/bard#BARD_0000313 24-well plate BARD> assay protocol> assay design> assay parameter> assay footprint> microplate> 24-well plate
Microplate containing 24 sample wells. Suitable for a low-throughput assay.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000576
316 http://www.bard.nih.gov/ontology/bard#BARD_0000314 3456-well plate BARD> assay protocol> assay design> assay parameter> assay footprint> microplate> 3456-well plate
Microplate containing 3456 sample wells. Suitable for a high-throughput assay.
317 http://www.bard.nih.gov/ontology/bard#BARD_0000315 384-well plate BARD> assay protocol> assay design> assay parameter> assay footprint> microplate> 384-well plate
Microplate containing 384 sample wells. Suitable for a high-throughput assay.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000515
318 http://www.bard.nih.gov/ontology/bard#BARD_0000316 48-well plate BARD> assay protocol> assay design> assay parameter> assay footprint> microplate> 48-well plate
Microplate containing 48 sample wells. Suitable for a low-throughput assay.
319 http://www.bard.nih.gov/ontology/bard#BARD_0000317 6-well plate BARD> assay protocol> assay design> assay parameter> assay footprint> microplate> 6-well plate
Microplate containing 6 sample wells. Suitable for a low-throughput assay.
320 http://www.bard.nih.gov/ontology/bard#BARD_0000318 96-well plate BARD> assay protocol> assay design> assay parameter> assay footprint> microplate> 96-well plate
Microplate containing 96 sample wells. Suitable for a low-throughput assay.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000513
2309 http://bard.broadinstitute.org/bard#2309 Cell Boat BARD> assay protocol> assay design> assay parameter> assay footprint> microplate> Cell Boat
For IonWorks automated patch clamp
2299 http://bard.broadinstitute.org/bard#2299 QPlate16 BARD> assay protocol> assay design> assay parameter> assay footprint> microplate> QPlate16
For QPatch16 automated electrophysiology assay. Sophion Bioscience, Ballerup, Denmark
1428 http://www.bard.nih.gov/ontology/bard#BARD_0001601 microscope slide BARD> assay protocol> assay design> assay parameter> assay footprint> microscope slide
from UNM min Assay annnotaion xls
2388 http://bard.broadinstitute.org/bard#2388 16-chamber slide BARD> assay protocol> assay design> assay parameter> assay footprint> microscope slide> 16-chamber slide
16-chamber slide
159 http://www.bard.nih.gov/ontology/bard#BARD_0000157 vial BARD> assay protocol> assay design> assay parameter> assay footprint> vial
Small containers that are used in biological measurements, including scintillation counting.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000577
81 http://www.bard.nih.gov/ontology/bard#BARD_0000079 assay phase BARD> assay protocol> assay design> assay parameter> assay phase
A type of assay parameter that describes whether all the assay components are in solution or some are in solid phase, which determines their ability to scatter light.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000371
160 http://www.bard.nih.gov/ontology/bard#BARD_0000158 heterogeneous phase BARD> assay protocol> assay design> assay parameter> assay phase> heterogeneous phase
One or more assay components are present in solid phase at time of detection (e.g., cells, beads). The components in solid phase can scatter light.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000373
161 http://www.bard.nih.gov/ontology/bard#BARD_0000159 homogeneous phase BARD> assay protocol> assay design> assay parameter> assay phase> homogeneous phase
All assay components exist in solution phase at the time of detection (e.g., none of the components are in beads or cells). Technically no component scatters light.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0000372
280 http://www.bard.nih.gov/ontology/bard#BARD_0000278 solid phase BARD> assay protocol> assay design> assay parameter> assay phase> homogeneous phase> solid phase
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002163
281 http://www.bard.nih.gov/ontology/bard#BARD_0000279 solution phase BARD> assay protocol> assay design> assay parameter> assay phase> homogeneous phase> solution phase
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002164
82 http://www.bard.nih.gov/ontology/bard#BARD_0000080 cell-processing method BARD> assay protocol> assay design> assay parameter> cell-processing method
A type of assay parameter that is the development and manufacture of innovative cell based therapies used for clinical testing.
Open Biomedical Ontologies : http://purl.obolibrary.org/obo/ID_0000055
169 http://www.bard.nih.gov/ontology/bard#BARD_0000167 cell fixation method BARD> assay protocol> assay design> assay parameter> cell-processing method> cell fixation method
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002035
170 http://www.bard.nih.gov/ontology/bard#BARD_0000168 labeling method BARD> assay protocol> assay design> assay parameter> cell-processing method> labeling method
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002452
301 http://www.bard.nih.gov/ontology/bard#BARD_0000299 colorimetric labeling method BARD> assay protocol> assay design> assay parameter> cell-processing method> labeling method> colorimetric labeling method
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002453
372 http://www.bard.nih.gov/ontology/bard#BARD_0000370 colorimetric cell-staining method BARD> assay protocol> assay design> assay parameter> cell-processing method> labeling method> colorimetric labeling method> colorimetric cell-staining method
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002455
373 http://www.bard.nih.gov/ontology/bard#BARD_0000371 colorimetric protein-staining method BARD> assay protocol> assay design> assay parameter> cell-processing method> labeling method> colorimetric labeling method> colorimetric protein-staining method
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002462
374 http://www.bard.nih.gov/ontology/bard#BARD_0000372 crystal violet staining method BARD> assay protocol> assay design> assay parameter> cell-processing method> labeling method> colorimetric labeling method> crystal violet staining method
Crystal violet is a dye derived from gentian violet that is used as a general biological stain and an acid-base indicator.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002468
302 http://www.bard.nih.gov/ontology/bard#BARD_0000300 fluorescent labeling method BARD> assay protocol> assay design> assay parameter> cell-processing method> labeling method> fluorescent labeling method
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002469
398 http://www.bard.nih.gov/ontology/bard#BARD_0000396 fluorescent DNA hybridization probe method BARD> assay protocol> assay design> assay parameter> cell-processing method> labeling method> fluorescent labeling method> fluorescent DNA hybridization probe method
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002470
399 http://www.bard.nih.gov/ontology/bard#BARD_0000397 fluorescent DNA intercalation method BARD> assay protocol> assay design> assay parameter> cell-processing method> labeling method> fluorescent labeling method> fluorescent DNA intercalation method
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002472
400 http://www.bard.nih.gov/ontology/bard#BARD_0000398 fluorescent dye transfer method BARD> assay protocol> assay design> assay parameter> cell-processing method> labeling method> fluorescent labeling method> fluorescent dye transfer method
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002477
303 http://www.bard.nih.gov/ontology/bard#BARD_0000301 immunolabeling method BARD> assay protocol> assay design> assay parameter> cell-processing method> labeling method> immunolabeling method
A means of localizing particular antigens within tissue. Immunolabeling is a methodological process where: antibodies are used to identify antigens within an organism and a tag (e.g., through fluorescence, gold beads, epitope tag, etc.) is added to a secondary antibody which binds to the primary antibody.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002425
403 http://www.bard.nih.gov/ontology/bard#BARD_0000401 immunofluorescent labeling method BARD> assay protocol> assay design> assay parameter> cell-processing method> labeling method> immunolabeling method> immunofluorescent labeling method
The labeling of antibodies or antigens with fluorescent dyes.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002426
404 http://www.bard.nih.gov/ontology/bard#BARD_0000402 immunogold labeling method BARD> assay protocol> assay design> assay parameter> cell-processing method> labeling method> immunolabeling method> immunogold labeling method
The identification of an antigen by conjugating gold particles to a primary or secondary antibody.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002427
304 http://www.bard.nih.gov/ontology/bard#BARD_0000302 isotope labeling method BARD> assay protocol> assay design> assay parameter> cell-processing method> labeling method> isotope labeling method
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002478
305 http://www.bard.nih.gov/ontology/bard#BARD_0000303 photoaffinity labeling method BARD> assay protocol> assay design> assay parameter> cell-processing method> labeling method> photoaffinity labeling method
Technology that attaches a label to the active site of a large molecule, especially a protein. Uses a molecule that binds loosely and reversibly to the large molecule's active site and has an inactive part that can be converted by photolysis to a highly reactive form. This causes the complex of the large substrate molecule and the loosely attached label to bind more permanently.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002479
306 http://www.bard.nih.gov/ontology/bard#BARD_0000304 vital dye staining method BARD> assay protocol> assay design> assay parameter> cell-processing method> labeling method> vital dye staining method
Employs a stain that is capable of entering and staining a living cell without causing an injury.
BioAssay Ontology : http://www.bioassayontology.org/bao#BAO_0002480
427 http://www.bard.nih.gov/ontology/bard#BARD_0000426